大鼠肝脏线粒体伴随短链脂肪酸氧化的氧化磷酸化作用。
Oxidative phosphorylation accompanying oxidation of short-chain fatty acids by rat-liver mitochondria.
作者信息
Hird F J, Weidemann M J
出版信息
Biochem J. 1966 Feb;98(2):378-88. doi: 10.1042/bj0980378.
Abstract
- The factors concerned in the estimation of P/O ratios when fatty acids are oxidized by rat-liver mitochondria have been assessed. 2. The oxidation of butyrate, hexanoate and octanoate is accompanied by ATP synthesis. At low concentrations of the fatty acids, P/O ratios approximately 2.5 are obtained. 3. Oxidative phosphorylation is uncoupled, respiratory control ratios are lowered and respiration is inhibited when the concentration of the fatty acid in the incubating medium is raised (to 5-10mm); octanoate is a more potent uncoupler than either hexanoate or butyrate. 4. Serum albumin and carnitine, either singly or in combination, protect the mitochondria from the effect exerted by the fatty acids. 5. The rate of oxidation of short-chain fatty acids in the presence of ADP is increased in the presence of carnitine.
摘要
- 对大鼠肝脏线粒体氧化脂肪酸时P/O比值估算中涉及的因素进行了评估。2. 丁酸、己酸和辛酸的氧化伴随着ATP的合成。在脂肪酸浓度较低时，可得到约为2.5的P/O比值。3. 当孵育介质中脂肪酸浓度升高（至5 - 10mM）时，氧化磷酸化解偶联，呼吸控制率降低，呼吸受到抑制；辛酸比己酸或丁酸更有效地解偶联。4. 血清白蛋白和肉碱单独或联合使用可保护线粒体免受脂肪酸的影响。5. 在有ADP存在的情况下，肉碱可提高短链脂肪酸的氧化速率。
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