Bartsch G G, Gerber G B
J Lipid Res. 1966 Mar;7(2):204-9.
In order to study lipid metabolism in the liver without interference due to transport from and to the liver the isolated livers of normal, X-irradiated, and partially hepatectomized rats were perfused with acetate-1-(14)C and the distribution of radioactivity in total lipids, total fatty acids, individual lipids, and fatty acids of individual lipids was determined. In X-irradiated animals, an increased incorporation of acetate into many lipids, particularly into cholesterol, was observed. Lipids in the liver of the partially hepatectomized rats exhibited a marked increase in triglyceride content together with a decreased rate of incorporation into all but the phospholipid fractions. It is concluded that the increase usually observed in lipid pontent of the regenerating liver is due to the changes in transcort rather than to changes in synthesis. The changes observed in irradiated liver could be the result of alterations in the metabolism of precursors common to most lipids.
为了在不受肝脏输入和输出干扰的情况下研究肝脏中的脂质代谢，用醋酸-1-(14)C灌注正常、经X射线照射和部分肝切除的大鼠的离体肝脏，并测定放射性在总脂质、总脂肪酸、个别脂质以及个别脂质的脂肪酸中的分布。在经X射线照射的动物中，观察到醋酸掺入许多脂质，特别是胆固醇的量增加。部分肝切除大鼠肝脏中的脂质，甘油三酯含量显著增加，同时除磷脂部分外，掺入所有其他部分的速率降低。得出的结论是，再生肝脏中通常观察到的脂质含量增加是由于转运变化而非合成变化。在受照射肝脏中观察到的变化可能是大多数脂质共同前体代谢改变的结果。
