Tomsak R L, Cook R T
Br J Cancer. 1977 Jun;35(6):713-21. doi: 10.1038/bjc.1977.112.
The in vitro metabolisms of [14C]7,12-dimethylbenz(a)anthracene (DMBA) by post-mitochondrial supernates and microsomes from intact and regenerating rat livers were compared. Both cell fractions from regenerating livers at 48, 72, and 96 h after partial hepatectomy metabolized less [14C]DMBA than similar fractions from intact livers. Prior in vivo treatment with DMBA enhanced metabolism by the cell fractions from both groups, but specific activities of cell fractions from regenerating livers were always about 60% or less of those from intact livers. Thin-layer chromatographic analysis of metabolites formed in incubations using either cell fraction failed to reveal distinct differences between ether-soluble or water-soluble products of similar fractions from intact and regenerating livers. However, highly reproducible differences were found between chromatograms of water-soluble metabolites formed by microsomes and post-mitochondrial supernates in both intact and regenerating livers. Extrapolations from these studies indicate large differences in the metabolic capacity of intact and regenerating livers when expressed on a whole-liver basis, but it is suggested that there may be additional factors contributing to the increased retention of DMBA by regenerating livers.
比较了来自完整大鼠肝脏和再生大鼠肝脏的线粒体后上清液和微粒体对[14C]7,12-二甲基苯并(a)蒽（DMBA）的体外代谢情况。部分肝切除术后48、72和96小时的再生肝脏的两种细胞组分代谢的[14C]DMBA比来自完整肝脏的类似组分少。之前用DMBA进行体内处理增强了两组细胞组分的代谢，但再生肝脏细胞组分的比活性始终约为完整肝脏细胞组分的60%或更低。使用任一细胞组分进行孵育时形成的代谢物的薄层色谱分析未能揭示完整肝脏和再生肝脏类似组分的醚溶性或水溶性产物之间的明显差异。然而，在完整肝脏和再生肝脏中，微粒体和线粒体后上清液形成的水溶性代谢物的色谱图之间发现了高度可重复的差异。这些研究的推断表明，以全肝为基础表示时，完整肝脏和再生肝脏的代谢能力存在很大差异，但有人认为可能还有其他因素导致再生肝脏对DMBA的保留增加。
