O'Hara D D, Porte D, Williams R H
J Lipid Res. 1966 Mar;7(2):264-9.
Fat particles (lipoproteins of S(f) > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v). As the concentration of PVP increased, a greater proportion of the particles accumulated at the top of the tube ("primary" particles). When the concentration of PVP in the tube was held constant at 3% PVP, dilution of the sample with plasma was found to produce an increased proportion of bottom particles ("secondary" particles). This observation suggests that bottom particles result from an interaction of top particles with plasma.
已从犬类和人类的淋巴以及人类餐后血浆中获取了脂肪颗粒（S(f) > 400的脂蛋白）。它们在5%聚乙烯吡咯烷酮（PVP）于10%氯化钠中的溶液中发生絮凝，并分层置于浓度在2%至5%（w/v）之间的不同PVP管底部。随着PVP浓度的增加，更大比例的颗粒聚集在管顶部（“初级”颗粒）。当管中PVP浓度保持在3% PVP不变时，发现用血浆稀释样品会使底部颗粒（“次级”颗粒）的比例增加。这一观察结果表明，底部颗粒是由顶部颗粒与血浆相互作用产生的。
