盘基网柄菌海藻糖酶：纯化及性质

Science. 1966 Jan 28;151(3709):454-6. doi: 10.1126/science.151.3709.454.

The hydrolytic enzyme, trehalase, was isolated and purified approximately 90-fold from the cellular slime mold Dictyostelium discoideum. The purified trehalase has an optimal temperature of 45 degrees C and shows maximum activity at pH 5.5 in citrate buffer. Its Michaelis constant is 1.2x 10(-3)M.

水解酶海藻糖酶从细胞黏菌盘基网柄菌中分离并纯化，纯化倍数约为90倍。纯化后的海藻糖酶最适温度为45℃，在柠檬酸盐缓冲液中pH 5.5时表现出最大活性。其米氏常数为1.2×10⁻³M。