形态正常和异常的公牛精子头部的DNA含量、干质量及光学面积研究。
Studies on the DNA content, dry mass and optical area of morphologically normal and abnormal bull spermatozoal heads.
作者信息
Gledhill B L
出版信息
Acta Vet Scand. 1966;7(1):1-20. doi: 10.1186/BF03547094.
Quantitative microspectrophotometric, microinterferometric and microplanimetric techniques were used to compare morphologically normal and abnormal bull spermatozoal heads with respect to DNA content, total dry mass and optical area. Spermatozoa were obtained from ejaculates of two young bulls with disturbances in spermatogenesis. U. v. microspectrophotometric results exhibited no difference in mean amounts of DNA between normal and abnormal spermatozoal heads. Apparently contradictory results were found with Feulgen microspectrophotometry and were discussed. Highly significant differences in mean values were observed between the normal and abnormal heads for total dry mass and optical area. The only cytophotometric parameter in which a significant difference existed between the variances of the two morphological groups was optical area.
运用定量显微分光光度法、显微干涉测量法和显微平面测量法，对形态正常和异常的公牛精子头部的DNA含量、总干质量和光学面积进行比较。精子取自两头精子发生有障碍的年轻公牛的射精样本。紫外显微分光光度法的结果显示，正常和异常精子头部的平均DNA含量没有差异。福尔根显微分光光度法得出了明显矛盾的结果，并对此进行了讨论。正常和异常头部在总干质量和光学面积的平均值上存在高度显著差异。两个形态学组的方差之间存在显著差异的唯一细胞光度学参数是光学面积。
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