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宽鳍花鳉体内的皮质醇:其鉴定及血浆中皮质醇测定方法的验证

Cortisol in Poecilia latipinna: its identification and the validation of methods for its determination in plasma.

作者信息

Hargreaves G, Ball J N

出版信息

Steroids. 1977 Sep;30(3):303-13. doi: 10.1016/0039-128x(77)90022-8.

DOI:10.1016/0039-128x(77)90022-8
PMID:595031
Abstract

In vitro studies in which head kidney of Poecilia latipinna was incubated with labelled precursors have shown that cortisol is the only corticosteroid that could be detected as being produced by this tissue. Cortisol levels have been measured in the plasma of Poecilia latipinna by three methods. The routine use of two rapid and comparatively simple methods, the competitive protein binding assay and the radioimmunoassay, have been validated in terms of the more rigorous double isotope dilution derivative assay.

摘要

在将帆鳉的头肾与标记前体一起孵育的体外研究中,已表明皮质醇是唯一可检测到由该组织产生的皮质类固醇。已通过三种方法测量了帆鳉血浆中的皮质醇水平。两种快速且相对简单的方法,即竞争性蛋白结合测定法和放射免疫测定法的常规使用,已根据更严格的双同位素稀释衍生测定法得到验证。

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Cortisol in Poecilia latipinna: its identification and the validation of methods for its determination in plasma.宽鳍花鳉体内的皮质醇:其鉴定及血浆中皮质醇测定方法的验证
Steroids. 1977 Sep;30(3):303-13. doi: 10.1016/0039-128x(77)90022-8.
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Rapid, specific assay for plasma cortisol by competitive protein binding.通过竞争性蛋白结合对血浆皮质醇进行快速、特异性检测。
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The determination of cortisol in fish plasma by radioimmunoassay.用放射免疫分析法测定鱼血浆中的皮质醇。
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[Preliminary evaluation cortisol determination kits by competitive protein binding analysis].[采用竞争性蛋白结合分析法对皮质醇测定试剂盒的初步评估]
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Studies on in-vivo cortisol secretion and its pituitary control in Poecilia latipinna (Teleostei).食蚊鱼(硬骨鱼纲)体内皮质醇分泌及其垂体调控的研究。
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Evaluation of a serum/plasma separator: suitability of samples for radioimmunoassay and competitive protein binding techniques.一种血清/血浆分离器的评估:样品用于放射免疫测定和竞争性蛋白结合技术的适用性。
Ann Clin Biochem. 1976 Jul;13(4):449-51. doi: 10.1177/000456327601300135.

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