用于血清和尿液中皮质醇测定的竞争性蛋白结合分析法与放射免疫分析法的比较。

Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine.

作者信息

Demers L M, Derck D D

出版信息

Clin Biochem. 1977 Jun;10(3):104-8. doi: 10.1016/s0009-9120(77)91392-3.

Abstract

The determination of serum and urinary free cortisol by competitive protein binding and radioimmunoassay were compared on patient samples using two commercially available kits. In addition, the Beckman RIA kit reagents were examined for precision and accuracy through the performance of reproducibility, recovery, cross reactivity and parallelism studies on serum and urine. Analytical recovery experiments with cortisol added to urine and serum showed 90% and 97% recovery respectively with the RIA reagents. Good reproducibility and parallelism criteria were also obtained using Beckman kit reagents. Antiserum specificity was documented and demonstrated no cross reactivity with structurally related steroids at clinically normal serum concentrations.

摘要

使用两种市售试剂盒，对患者样本采用竞争性蛋白结合法和放射免疫分析法测定血清和尿游离皮质醇进行了比较。此外，通过对血清和尿液进行重复性、回收率、交叉反应性和平行性研究，对贝克曼放射免疫分析试剂盒试剂的精密度和准确性进行了检验。向尿液和血清中添加皮质醇的分析回收率实验表明，使用放射免疫分析试剂时，回收率分别为90%和97%。使用贝克曼试剂盒试剂也获得了良好的重复性和平行性标准。记录了抗血清特异性，证明在临床正常血清浓度下与结构相关类固醇无交叉反应。