Sokolovskiĭ V V, Belozerova L A, Ogurtsova R E
Vopr Med Khim. 1977 Sep-Oct;23(5):709-12.
A method of reversed amperometric titration for estimation of content of S- S-groups in biological materials is described. The method is based on splitting of disulphide groups in reaction with a reducing agent (Na2SO3) in presence of an excess of metal ions (Ag+, Hg2+), which form a mercaptide bond (AgS) with SH-groups. The excess of free metal ions was titrated with the equimolar solution of thiol (unithiol, cysteine). The method is comparatively simple and enables to estimate S- S groups in presence of air oxygen and enzymatic systems.
描述了一种用于测定生物材料中S-S基团含量的反向安培滴定法。该方法基于在过量金属离子（Ag+、Hg2+）存在下，二硫键与还原剂（Na2SO3）反应断裂，金属离子与SH基团形成硫醇盐键（AgS）。用硫醇（二巯丙醇、半胱氨酸）的等摩尔溶液滴定过量的游离金属离子。该方法相对简单，能够在有氧和酶系统存在的情况下测定S-S基团。
