苯丙氨酸对短杆菌肽S形成的刺激作用。
Phenylalanine stimulation of gramicidin S formation.
作者信息
Demain A L, Matteo C C
出版信息
Antimicrob Agents Chemother. 1976 Jun;9(6):1000-3. doi: 10.1128/AAC.9.6.1000.
Bacillus brevis produces the antibiotic gramicidin S, a cyclic peptide comprised of two pentapeptide units of the sequence d-Phe-l-Pro-l-Val-l-Orn-l-Leu. Synthesis of this antibiotic is carried out by two enzymes, GS synthetases I and II. By supplementing a defined minimal medium [glycerol, NH(4) (+), tris(hydroxymethyl)aminomethane, salts] with each of the constituent amino acids (in the l form), we found that only l-phenylalanine had a marked stimulatory effect on gramicidin S production. This effect was not caused by an increase in growth, by an induction of GS synthetases I or II, or by a stabilization of these enzymes. l-Phenylalanine apparently stimulates production via its role as a precursor of the d-phenylalanine moiety of the gramicidin S molecule.
短短芽孢杆菌产生抗生素短杆菌肽S，它是一种环肽，由序列为d-苯丙氨酸-l-脯氨酸-l-缬氨酸-l-鸟氨酸-l-亮氨酸的两个五肽单元组成。这种抗生素的合成由两种酶，即短杆菌肽S合成酶I和II来完成。通过在确定的基本培养基[甘油、NH(4)+、三（羟甲基）氨基甲烷、盐类]中分别添加每种组成氨基酸（l型），我们发现只有l-苯丙氨酸对短杆菌肽S的产生有显著的刺激作用。这种作用不是由生长增加、短杆菌肽S合成酶I或II的诱导或这些酶的稳定化引起的。l-苯丙氨酸显然是通过作为短杆菌肽S分子中d-苯丙氨酸部分的前体来刺激产量的。
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