Sood P P
Z Parasitenkd. 1977 Nov 10;53(3):267-72. doi: 10.1007/BF00389943.
Butyryl cholinesterase activity in Glossimetra orientalis was studied histochemically with Gomori's method using butyrylthiocholine as substrate. Eserine sulphate (10(-5) M) was used as inhibitor for AChE. The study reveals that the enzyme is present mainly in the musculature of the reproductive system, excretory canal, nerve cells and fibers, tegument and subtegumentary cells and suckers. The testes, ovary and parenchyma are completely negative. The functional significance of the enzyme in the various locations have been discussed.
采用Gomori法,以丁酰硫代胆碱为底物,对东方舌蛭的丁酰胆碱酯酶活性进行了组织化学研究。硫酸毒扁豆碱(10⁻⁵ M)用作乙酰胆碱酯酶的抑制剂。研究表明,该酶主要存在于生殖系统的肌肉组织、排泄管、神经细胞和纤维、体表及皮下细胞以及吸盘。睾丸、卵巢和实质组织均呈完全阴性。文中讨论了该酶在不同部位的功能意义。