[金黄色葡萄球菌荚膜抗原的化学与免疫原性特性研究。2. 体外实验中荚膜抗原免疫原性特性的评估]

[Study of the chemical and immunogenic properties of the capsular Staphylococcus aureus antigen. 2. Evaluation of the immunogenic properties of the capsular antigen in experiments in vitro].

作者信息

Seltmann G, Prokhorov V Ia, Witte W, Richter B, Akatov A K

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1977 Sep(9):33-6.

Abstract

Albino mice were immunized with a purified capsular antigen isolated from the S. aureus strain 1193/74. The presence of specific anticapsular antibodies in the sera of animals were determined by two methods: 1) by conversion of diffuse growth of a homologous strain into compact one, and 2) by determination of opsonic index in phagocytosis of homologous staphylococci by human neutrophils. It was revealed that antibodies converting the microbical growth were absent in the sera of normal mice and reached the highest level after the second antigen injection; opsonins were present in the sera of normal mice in widely varying quantities; their maximal level was noted after the 3rd immunization.

摘要

用从金黄色葡萄球菌1193/74菌株分离出的纯化荚膜抗原免疫白化小鼠。通过两种方法测定动物血清中特异性抗荚膜抗体的存在：1）通过将同源菌株的扩散生长转化为紧密生长，以及2）通过测定人中性粒细胞对同源葡萄球菌吞噬作用中的调理指数。结果显示，正常小鼠血清中不存在使微生物生长转化的抗体，在第二次注射抗原后达到最高水平；正常小鼠血清中存在数量差异很大的调理素；在第三次免疫后观察到其最高水平。