Saxegaard F, Teige J
Acta Vet Scand. 1977;18(4):563-5. doi: 10.1186/BF03548421.
Several successful attempts have been made to induce swine dysentery in pigs using pure cultures of Treponema hyodysen-teriae 1971, 1972, 1973, 1975). In these studies, either conventional or specific-pathogen-free pigs were used. In the present study, 2 approximately 8 weeks old conventional pigs (Nos. 1 and 2) were purchased and fed the same basic ration as used by (1977). In addition, 10 % cod liver oil was incorporated in the diet at each feeding. After a feeding period of 25 days rectal swabs were applied and examined for the presence of spirochaetes. The pigs were then fed a 3 days old primary and pure culture of T. hyodysenteriae on TSA-S400 medium 1976). The culture originated from the colon of a pig with swine dysentery (Pig No. 4, 1977). Each pig received the agar contents of 5 petri dishes which were mixed with the food.
人们已经进行了几次成功的尝试,使用猪痢疾密螺旋体的纯培养物在猪身上诱发猪痢疾(1971年、1972年、1973年、1975年)。在这些研究中,使用了常规猪或无特定病原体猪。在本研究中,购买了2头约8周龄的常规猪(1号和2号),并给它们喂食与(1977年)相同的基础日粮。此外,每次喂食时在日粮中添加10%的鱼肝油。在25天的喂食期后,采集直肠拭子并检查是否存在螺旋体。然后给猪喂食在TSA - S400培养基上培养3天的猪痢疾密螺旋体初代纯培养物(1976年)。该培养物源自一头患有猪痢疾的猪的结肠(4号猪,1977年)。每头猪都接受了与食物混合的5个培养皿的琼脂内容物。