Anyaibe S I, Headings V E
Am J Hematol. 1977;2(3):307-15. doi: 10.1002/ajh.2830020313.
We present here a method for electrophoretic identification of hemoglobin variants in many individual erythrocytes simultaneously, which is made possible by instruments developed in our laboratory. The system spaces and aligns the erythrocytes along a common origin and prevents 'doubling' of cells. Unambiguous separation of different hemoglobins (Hbs) occurred from single erythrocytes, such as Hbs A, C, F. Staged micrographs showed that hemoglobins of differing mobility from a single cell arise from a single point source of hemoglobin. In principle this capability is applicable to other proteins and could facilitate investigation at a cellular level into a variety of questions pertaining to gene action.
我们在此介绍一种能够同时对多个个体红细胞中的血红蛋白变体进行电泳鉴定的方法，这得益于我们实验室研发的仪器得以实现。该系统能使红细胞沿着共同起点排列并防止细胞“重叠”。不同血红蛋白（如血红蛋白A、C、F）可从单个红细胞中得到明确分离。分阶段拍摄的显微照片显示，来自单个细胞的迁移率不同的血红蛋白源自单一的血红蛋白点源。原则上，这种能力适用于其他蛋白质，并且有助于在细胞水平上研究与基因作用相关的各种问题。
