Anyaibe S I, Headings V E
Biochem Genet. 1980 Jun;18(5-6):455-63. doi: 10.1007/BF00484394.
Inherited electrophoretic variations of hemoglobin, carbonic anhydrase, and glucose-6-phosphate dehydrogenase in individual erythrocytes were separated by electrophoresis in ultrathin agar gels. By staining the electropherograms with specific fluorescein-conjugated antibodies against hemoglobins, relative proportions of two hemoglobins within individual erythrocytes can be estimated. The findings suggested that the intracellular proportions of HbA and HbS in heterozygotes are heterogeneous within a given population of cells. By this method cells containing hemoglobin F (F cells) as well as a minor variant of hemoglobin F were identified. This tool potentially offers an approach to monitoring distribution of inherited variants in individual erythrocytes for a large number of proteins.
通过在超薄琼脂凝胶中进行电泳，分离单个红细胞中血红蛋白、碳酸酐酶和葡萄糖-6-磷酸脱氢酶的遗传性电泳变异。通过用针对血红蛋白的特异性荧光素偶联抗体对电泳图谱进行染色，可以估计单个红细胞内两种血红蛋白的相对比例。研究结果表明，在给定的细胞群体中，杂合子中HbA和HbS的细胞内比例是异质的。通过这种方法，鉴定出了含有血红蛋白F（F细胞）以及血红蛋白F的一个小变体的细胞。该工具可能为监测大量蛋白质在单个红细胞中遗传变异的分布提供一种方法。
