在人体中应用的直接巨噬细胞游走抑制试验。
A direct macrophage migration inhibition test applied in man.
作者信息
Teelken A W, Minderhoud J M
出版信息
Clin Exp Immunol. 1976 Apr;24(1):26-32.
A direct macrophage migration test is described, which proved to be useful for the detection of cellular hypersensitivity in man. We used a modification of the method described by Hughes & Paty (1972). In this method the MLR is abolished by 100 rad gamma-irradiation of the peritoneal exudate cells prior to pooling with human lymphocytes. Experiments with various intensity of irradiation, PPD, muscle antigen and encephalitogenic factor were performed to check this method. In a pilot study lymphocytes of patients with diseases of the central or peripheral nervous system or of muscle were tested. This proved that a hypersensitivity of Ef was present in various diseases of the CNS, while in muscle diseases positive tests were found using muscle antigen.
本文描述了一种直接巨噬细胞迁移试验，该试验被证明可用于检测人体的细胞超敏反应。我们采用了休斯和帕蒂（1972年）所描述方法的改良版。在此方法中，在将腹腔渗出细胞与人淋巴细胞混合之前，先用100拉德的γ射线照射使混合淋巴细胞反应（MLR）消除。进行了不同照射强度、结核菌素纯蛋白衍生物（PPD）、肌肉抗原和致脑炎因子的实验来检验该方法。在一项初步研究中，对患有中枢或周围神经系统疾病或肌肉疾病患者的淋巴细胞进行了检测。结果证明，在中枢神经系统的各种疾病中存在迟发性超敏反应（Ef），而在肌肉疾病中，使用肌肉抗原可检测到阳性试验结果。
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