辐射诱导溶酶体释放酶以及脂质过氧化物形成与酶释放的关系。
Release of enzymes from lysosomes by irradiation and the relation of lipid peroxide formation to enzyme release.
作者信息
Wills E D, Wilkinson A E
出版信息
Biochem J. 1966 Jun;99(3):657-66. doi: 10.1042/bj0990657.
Abstract
- Acid phosphatase, cathepsin and beta-glucuronidase are released from rat-liver lysosomes by irradiation in vitro. Enzyme release is detectable after a dose of 1krad and increases with dose up to 100krads. 2. Maximum radiation effects were observed when the lysosomes were kept for 20hr. at 4 degrees or 20 degrees after irradiation. 3. An atmosphere of nitrogen considerably decreases enzyme release from lysosomes. 4. Enzyme release is enhanced by ascorbic acid and decreased by vitamin E. 5. Irradiation causes formation of lipid peroxides in lysosomes, and enzyme release increases with lipid peroxide formation. 6. It is suggested that lipid peroxide formation leads to rupture of the lysosome membrane and allows release of the contained hydrolytic enzymes.
摘要
- 体外照射可使大鼠肝脏溶酶体释放酸性磷酸酶、组织蛋白酶和β-葡萄糖醛酸酶。剂量达1千拉德时可检测到酶的释放，且随着剂量增加至100千拉德，酶释放量也增加。2. 照射后将溶酶体在4℃或20℃保存20小时，观察到最大辐射效应。3. 氮气环境可显著减少溶酶体中酶的释放。4. 抗坏血酸可增强酶的释放，维生素E则可减少酶的释放。5. 照射导致溶酶体中脂质过氧化物的形成，且酶释放量随脂质过氧化物的形成而增加。6. 有人提出，脂质过氧化物的形成导致溶酶体膜破裂，使所含水解酶得以释放。
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本文引用的文献
1
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J Clin Invest. 1938 Jul;17(4):473-8. doi: 10.1172/JCI100974.
2
The thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acids by various agents.硫代巴比妥酸试剂用于检测各种试剂对不饱和脂肪酸的氧化作用。
Arch Biochem. 1949 Dec;24(2):305-13.
3
A microspectrophotometric method for the determination of cytochrome oxidase.一种测定细胞色素氧化酶的显微分光光度法。
J Biol Chem. 1951 Apr;189(2):665-70.
4
Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.组织分级分离研究。4. 大鼠肝脏颗粒对酸性磷酸酶、β-葡萄糖醛酸酶和组织蛋白酶结合的比较研究。
Biochem J. 1955 Mar;59(3):433-8. doi: 10.1042/bj0590433.
5
MECHANISMS OF LIPID PEROXIDE FORMATION IN TISSUES. ROLE OF METALS AND HAEMATIN PROTEINS IN THE CATALYSIS OF THE OXIDATION UNSATURATED FATTY ACIDS.组织中脂质过氧化物形成的机制。金属和血红素蛋白在催化不饱和脂肪酸氧化中的作用。
Biochim Biophys Acta. 1965 Apr 5;98:238-51. doi: 10.1016/0005-2760(65)90118-9.
6
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Int J Radiat Biol Relat Stud Phys Chem Med. 1964;8:551-67. doi: 10.1080/09553006414550701.
7
THE EFFECT OF INORGANIC IRON ON THE THIOBARBITURIC ACID METHOD FOR THE DETERMINATION OF LIPID PEROXIDES.无机铁对硫代巴比妥酸法测定脂质过氧化物的影响。
Biochim Biophys Acta. 1964 Aug 5;84:475-7. doi: 10.1016/0926-6542(64)90016-2.
8
PEROXIDATIVE AND RADIATION DAMAGE TO ISOLATED LYSOSOMES.分离的溶酶体的过氧化和辐射损伤
Biochim Biophys Acta. 1964 May 11;86:277-85. doi: 10.1016/0304-4165(64)90054-6.
9
ROLE OF ASCORBATE AND CYSTEINE ON SWELLING AND LIPID PEROXIDATION IN RAT LIVER MITOCHONDRIA.抗坏血酸盐和半胱氨酸对大鼠肝脏线粒体肿胀及脂质过氧化的作用
Arch Biochem Biophys. 1964 Feb;104:241-7. doi: 10.1016/s0003-9861(64)80010-2.
10
LYSOSOME PARTICLES AND SUBCELLULAR DISTRIBUTIONS OF POLYMERIC TETRAVALENT PLUTONIUM-239.聚合四价钚-239的溶酶体颗粒及亚细胞分布
Radiat Res. 1964 Apr;21:575-83.
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