在叶绿体发育过程中[14C]二氧化碳和[2-14C]甲羟戊酸掺入高等植物萜类化合物的过程。
Incorporation of [14C] carbon dioxide and [2-14C] mevalonic acid into terpenoids of higher plants during chloroplast development.
作者信息
Treharne K J, Mercer E I, Goodwin T W
出版信息
Biochem J. 1966 Apr;99(1):239-45. doi: 10.1042/bj0990239.
Abstract
- The incorporation of (14)CO(2) and dl-[2-(14)C]mevalonic acid into various terpenoids in developing chloroplasts in a number of seedlings has been studied. 2. beta-Carotene and phytol (from chlorophyll) tend to be heavily labelled from (14)CO(2), whereas sterols and beta-amyrin are only slightly labelled; with dl-[2-(14)C]mevalonic acid the situation is reversed. 3. The incorporation of (14)CO(2) into terpenoids is dependent on the stage of chloroplast development, whereas that of mevalonic acid is independent of chloroplast development. 4. The uptake of (14)CO(2) into beta-carotene and phytol in mature chloroplasts is very low in monocotyledons but somewhat greater in dicotyledons. 5. The results are discussed in relation to the view that terpenoid biosynthesis in developing chloroplasts is regulated by a combination of enzyme segregation and specific membrane permeability.
摘要
- 已经研究了在多种幼苗发育中的叶绿体中，（14）CO₂和dl-[2-（14）C]甲羟戊酸掺入各种萜类化合物的情况。2. β-胡萝卜素和叶绿醇（来自叶绿素）倾向于从（14）CO₂中大量标记，而甾醇和β-香树精仅被轻微标记；使用dl-[2-（14）C]甲羟戊酸时情况则相反。3. （14）CO₂掺入萜类化合物取决于叶绿体发育阶段，而甲羟戊酸的掺入则与叶绿体发育无关。4. 在成熟叶绿体中，单子叶植物中（14）CO₂进入β-胡萝卜素和叶绿醇的量非常低，而在双子叶植物中则略高。5. 结合叶绿体发育过程中萜类生物合成受酶分离和特定膜通透性共同调节的观点对结果进行了讨论。
相似文献
1
Incorporation of [14C] carbon dioxide and [2-14C] mevalonic acid into terpenoids of higher plants during chloroplast development.在叶绿体发育过程中[14C]二氧化碳和[2-14C]甲羟戊酸掺入高等植物萜类化合物的过程。
Biochem J. 1966 Apr;99(1):239-45. doi: 10.1042/bj0990239.
2
Incorporation of DL-[2-14C]mevalonic acid lactone into beta-carotene and the phytol side chain of chlorophyll in cotyledons of four species of pine seedlings.DL-[2-¹⁴C]甲羟戊酸内酯掺入四种松树幼苗子叶中β-胡萝卜素及叶绿素的叶绿醇侧链的情况。
Biochem J. 1967 Oct;105(1):89-92. doi: 10.1042/bj1050089.
3
Incorporation of 2-[14C]mevalonic acid into phytoene by isolated chloroplasts.分离的叶绿体将2-[14C]甲羟戊酸掺入八氢番茄红素的过程。
Biochem J. 1967 Oct;105(1):205-12. doi: 10.1042/bj1050205.
4
Biosynthesis of abscisic acid: incorporation of radioactivity from [2-14C]mevalonic acid by intact fruit.脱落酸的生物合成：完整果实对[2-¹⁴C]甲瓦龙酸中放射性的掺入。
Biochem J. 1969 May;112(4):547-8. doi: 10.1042/bj1120547.
5
Biosynthesis of the prenyl side chains of plastoquinone and related compounds in maize and barley shoots.玉米和大麦嫩枝中叶醌及相关化合物异戊烯基侧链的生物合成
Biochem J. 1967 Jun;103(3):831-51. doi: 10.1042/bj1030831.
6
Intracellular localization of mevalonate-activating enzymes in plant cells.甲羟戊酸激活酶在植物细胞中的细胞内定位。
Biochem J. 1966 May;99(2):381-8. doi: 10.1042/bj0990381.
7
Observations on the nature and biosynthesis of terpenoid quinones and related compounds in tobacco shoots.烟草嫩枝中萜类醌及相关化合物的性质与生物合成研究
Eur J Biochem. 1968 Jun;5(1):124-32. doi: 10.1111/j.1432-1033.1968.tb00346.x.
8
Sterol and triterpene synthesis in the developing and germinating pea seed.发育中和萌发中的豌豆种子中的甾醇和三萜合成
Biochem J. 1971 Sep;124(2):375-83. doi: 10.1042/bj1240375.
9
Biosynthesis of chloroplastidic and extrachloroplastidic terpenoids in liverwort cultured cells: 13C serine as a probe of terpene biosynthesis via mevalonate and non-mevalonate pathways.苔类培养细胞中叶绿体和叶绿体外萜类化合物的生物合成：¹³C丝氨酸作为通过甲羟戊酸途径和非甲羟戊酸途径进行萜类生物合成的探针。
J Nat Prod. 2003 Mar;66(3):332-6. doi: 10.1021/np0204141.
10
Compartmentation of terpenoid biosynthesis in green plants. A proposed route of acetyl-coenzyme A synthesis in maize chloroplasts.绿色植物中萜类生物合成的区室化。玉米叶绿体中乙酰辅酶A合成的一条拟议途径。
Biochem J. 1969 Sep;114(2):395-405. doi: 10.1042/bj1140395.
引用本文的文献
1
Metabolic Origins and Transport of Vitamin E Biosynthetic Precursors.维生素E生物合成前体的代谢起源与转运
Front Plant Sci. 2017 Nov 14;8:1959. doi: 10.3389/fpls.2017.01959. eCollection 2017.
2
Events Surrounding the Early Development of Euglena Chloroplasts: 7. Inhibition of Carotenoid Biosynthesis by the Herbicide SAN 9789 (4-Chloro-5-(methylamino)-2-(alpha,alpha,alpha,-trifluoro-m-tolyl)-3-(2H)pyridazinone) and Its Developmental Consequences.眼虫叶绿体早期发育相关事件：7. 除草剂 SAN 9789（4-氯-5-（甲氨基）-2-（α，α，α-三氟-间-甲苯基）-3-（2H）哒嗪酮）对类胡萝卜素生物合成的抑制及其发育后果。
Plant Physiol. 1976 Feb;57(2):260-9. doi: 10.1104/pp.57.2.260.
3
Mevalonate-derived isopentenyl diphosphate is the biosynthetic precursor of ubiquinone prenyl side chain in tobacco BY-2 cells.甲羟戊酸衍生的异戊烯基二磷酸是烟草BY-2细胞中泛醌异戊烯基侧链的生物合成前体。
Biochem J. 1998 Apr 15;331 ( Pt 2)(Pt 2):615-21. doi: 10.1042/bj3310615.
4
Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus.通过斜生栅藻中一条新的丙酮酸/3-磷酸甘油醛非甲羟戊酸途径合成类异戊二烯（类胡萝卜素、甾醇、叶绿素的异戊烯侧链和质体醌）。
Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):73-80. doi: 10.1042/bj3160073.
5
Incorporation of DL-[2-14C]mevalonic acid lactone into beta-carotene and the phytol side chain of chlorophyll in cotyledons of four species of pine seedlings.DL-[2-¹⁴C]甲羟戊酸内酯掺入四种松树幼苗子叶中β-胡萝卜素及叶绿素的叶绿醇侧链的情况。
Biochem J. 1967 Oct;105(1):89-92. doi: 10.1042/bj1050089.
6
Biosynthesis of the prenyl side chains of plastoquinone and related compounds in maize and barley shoots.玉米和大麦嫩枝中叶醌及相关化合物异戊烯基侧链的生物合成
Biochem J. 1967 Jun;103(3):831-51. doi: 10.1042/bj1030831.
7
Mevalonate-kinase isoenzymes in plant cells.植物细胞中的甲羟戊酸激酶同工酶。
Biochem J. 1966 Aug;100(2):14contd-17c. doi: 10.1042/bj1000014c.
8
The sterol esters of maize seedlings.玉米幼苗的甾醇酯
Biochem J. 1968 Nov;110(1):111-8. doi: 10.1042/bj1100111.
9
The pattern and control of isoprenoid quinone and tocopherol metabolism in the germinating grain of wheat (Triticum vulgare).小麦（普通小麦）萌发种子中类异戊二烯醌和生育酚代谢的模式与调控。
Biochem J. 1968 Jul;108(3):475-82. doi: 10.1042/bj1080475.
10
Compartmentation of terpenoid biosynthesis in green plants. A proposed route of acetyl-coenzyme A synthesis in maize chloroplasts.绿色植物中萜类生物合成的区室化。玉米叶绿体中乙酰辅酶A合成的一条拟议途径。
Biochem J. 1969 Sep;114(2):395-405. doi: 10.1042/bj1140395.
本文引用的文献
1
Studies in carotenogenesis. 29. Attempts to detect lycopersene in higher plants.类胡萝卜素生成的研究。29. 检测高等植物中番茄红素的尝试。
Biochem J. 1963 May;87(2):317-25. doi: 10.1042/bj0870317.
2
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
3
Purification of chlorophylls, pheophytins and pheophorbides for specific activity determinations.用于比活性测定的叶绿素、脱镁叶绿素和脱镁叶绿酸的纯化。
Biochim Biophys Acta. 1962 Apr 23;58:486-98. doi: 10.1016/0006-3002(62)90059-8.
Zotero 插件