Sowerby J M, Ottaway J H
Biochem J. 1966 Apr;99(1):246-52. doi: 10.1042/bj0990246.
A method of estimating glutamic acid is described, based on its dehydrogenation by glutamate dehydrogenase coupled, by means of N-methylphenazine methosulphate, to the reduction of tetrazolium salts. The method is suitable for the estimation of 0-0.3mumole of glutamic acid. The response is linear, but not stoicheiometric: possible reasons for this are discussed. If suitable precautions are taken, the use of a partially purified preparation of glutaminase makes it possible to estimate glutamine also.
本文描述了一种估算谷氨酸的方法，该方法基于谷氨酸脱氢酶对谷氨酸的脱氢作用，并借助硫酸N - 甲基吩嗪将其与四氮唑盐的还原反应偶联。该方法适用于估算0 - 0.3微摩尔的谷氨酸。反应呈线性，但不符合化学计量关系：文中讨论了可能的原因。如果采取适当的预防措施，使用部分纯化的谷氨酰胺酶制剂也能够估算谷氨酰胺。
