Modified metabolic-inhibition test for detection of antibodies to Mycoplasma hyosynoviae in swine serum.

A modification of the metabolic inhibition (MI) test was used for detection of antibodies against Mycoplasma hyosynoviae in convalescent swine serum. Supplementation of the MI system with 1% unheated normal rabbit serum, as well as 6% unheated normal guinea pig serum was required for detection of MI antibodies in early phase, postinfection serum and markedly improved detection of MI antibodies in late phase, postinfection serum. Factor(s) supplies by rabbit serum was not supplied by unheated normal swine serum.