Kazama S, Yagihashi T, Seto K
Nippon Institute for Biological Science, Tokyo, Japan.
Can J Vet Res. 1989 Apr;53(2):176-81.
Methods of preparation of Mycoplasma hyopneumoniae antigens for the enzyme-linked immunosorbent assay to detect specific antibody, and properties of the antigens, are described. The reactivity and specificity of antigen prepared by Sephacryl S-300 column chromatography after treatment of M. hyopneumoniae cells with Tween 20 (S-300 antigen) were superior to those of antigen prepared by Sephadex G-25 column chromatography after treatment with Tween 20, or to lipid antigen. There were no differences among strains MI-3, J and VPP11 of M. hyopneumoniae. The S-300 antigen did not show cross-reactivity against porcine hyperimmune sera produced by M. hyorhinis, M. hyosynoviae, M. hyopharyngis, M. flocculare and Acholeplasma granularum. Antibody was first detected in sera of pigs inoculated intranasally with M. hyopneumoniae at two to four weeks after inoculation and seven to eight weeks after pigs were contact-exposed to the same mycoplasma.
本文描述了用于酶联免疫吸附测定以检测特异性抗体的猪肺炎支原体抗原的制备方法及其抗原特性。用吐温20处理猪肺炎支原体细胞后，通过Sephacryl S - 300柱色谱法制备的抗原（S - 300抗原）的反应性和特异性优于用吐温20处理后通过Sephadex G - 25柱色谱法制备的抗原或脂质抗原。猪肺炎支原体的MI - 3、J和VPP11菌株之间没有差异。S - 300抗原与由猪鼻支原体、猪滑膜支原体、猪咽支原体、絮状支原体和颗粒无胆甾原体产生的猪超免疫血清没有交叉反应。在经鼻接种猪肺炎支原体的猪血清中，接种后两到四周以及猪与相同支原体接触暴露后七到八周首次检测到抗体。
