甲状腺淋巴液和血液中的放射性碘蛋白。
Radioactive iodoprotein in thyroid lymph and blood.
作者信息
Daniel P M, Plaskett L G, Pratt O E
出版信息
Biochem J. 1966 Sep;100(3):622-30. doi: 10.1042/bj1000622.
Abstract
- Samples of thyroid and non-thyroid lymph and of thyroid and peripheral venous blood were obtained from primates and cats that had previously been given radioactive iodine. The distribution of the organic radioiodine between the protein and non-protein fractions in these samples was determined. 2. The proportion of the organic radioiodine in the form of iodoprotein was assessed by paper chromatography, acid-ethanol precipitation, hot-butanol washing, column chromatography and separate estimation of iodotyrosines after enzymic hydrolysis. 3. In thyroid lymph the relative proportion of the organic radioiodine in the form of iodoprotein was 75-98%; in blood it was much lower, probably no more than 6-7%. The absolute concentration of iodoprotein radioactivity also was considerably greater in thyroid lymph than in blood. 4. Enzymic hydrolysis of the protein of the thyroid lymph yielded a pattern of iodoamino acids that corresponded closely with that obtained after hydrolysis of protein extracted from the thyroid gland itself. 5. It can be concluded that the iodoprotein in thyroid lymph consists mainly of thyroglobulin or a closely related compound.
摘要
- 从先前已给予放射性碘的灵长类动物和猫身上获取甲状腺和非甲状腺淋巴样本以及甲状腺和外周静脉血样本。测定这些样本中有机放射性碘在蛋白质和非蛋白质部分之间的分布。2. 通过纸色谱法、酸乙醇沉淀法、热丁醇洗涤法、柱色谱法以及酶水解后对碘酪氨酸的单独测定，评估以碘蛋白形式存在的有机放射性碘的比例。3. 在甲状腺淋巴中，以碘蛋白形式存在的有机放射性碘的相对比例为75% - 98%；在血液中则低得多，可能不超过6% - 7%。甲状腺淋巴中碘蛋白放射性的绝对浓度也明显高于血液中的。4. 甲状腺淋巴蛋白质的酶水解产生的碘氨基酸模式与从甲状腺本身提取的蛋白质水解后得到的模式密切对应。5. 可以得出结论，甲状腺淋巴中的碘蛋白主要由甲状腺球蛋白或密切相关的化合物组成。
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本文引用的文献
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Nature of the circulating thyroid hormone.循环甲状腺激素的性质。
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Radioactive iodine in the lymph leaving the thyroid gland.离开甲状腺的淋巴液中的放射性碘。
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Iodoprotein in the thyroid lymph of primates.灵长类动物甲状腺淋巴中的碘蛋白。
Nature. 1963 Apr 27;198:392-3. doi: 10.1038/198392a0.
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