用胰酶、链霉蛋白酶和胃蛋白酶水解125-I标记的甲状腺球蛋白。
Hydrolysis of 125-I-labelled thyroglobulin by pancreatin, pronase and pepsin.
作者信息
Malan P G
出版信息
Biochem J. 1968 Oct;109(5):787-92. doi: 10.1042/bj1090787.
Abstract
- Pancreatin or Pronase hydrolysis of partially purified heat-denatured (125)I-labelled thyroglobulin does not release all the iodoamino acids from peptide linkage. 2. The addition of thyroidal proteases is essential to obtain maximum hydrolysis of thyroglobulin. 3. Pronase plus a fresh non-radioactive thyroid homogenate gives the best hydrolysis results, but peptide ;mapping' shows that about 10% of the radioactivity remains in the form of peptides. 4. These peptides have approximately the same R(F) as the iodotyrosines on one-dimensional chromatograms developed in butan-1-ol-2n-acetic acid (1:1, v/v). 5. Peptic hydrolysis of thyroglobulin releases virtually all of the iodothyronines but little of the iodotyrosines, in contrast with the action of pancreatin. 6. Hydrolysis of thyroglobulin with pepsin followed by Pronase plus a source of thyroidal proteases is satisfactory, but the results are not superior to those obtained by using Pronase plus a source of thyroidal proteases.
摘要
- 胰酶或链霉蛋白酶对部分纯化的热变性（125）I标记甲状腺球蛋白的水解作用，不能使所有碘氨基酸从肽键中释放出来。2. 添加甲状腺蛋白酶对于实现甲状腺球蛋白的最大程度水解至关重要。3. 链霉蛋白酶加新鲜的非放射性甲状腺匀浆可产生最佳水解结果，但肽“图谱分析”表明，约10%的放射性仍以肽的形式存在。4. 在1-丁醇-2N-乙酸（1:1，v/v）展开的一维色谱图上，这些肽的R（F）与碘酪氨酸大致相同。5. 与胰酶的作用相反，甲状腺球蛋白的胃蛋白酶水解几乎释放出所有的碘甲状腺原氨酸，但仅释放少量碘酪氨酸。6. 先用胃蛋白酶水解甲状腺球蛋白，再用链霉蛋白酶加甲状腺蛋白酶来源进行水解是令人满意的，但结果并不优于使用链霉蛋白酶加甲状腺蛋白酶来源所获得的结果。
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