从人垂体中分离和部分纯化蛋白质激素。
Separation and partial purification of the protein hormones from human pituitary glands.
作者信息
Hartree A S
出版信息
Biochem J. 1966 Sep;100(3):754-61. doi: 10.1042/bj1000754.
Abstract
A method has been developed for separation and partial purification of four protein hormones from acetone-dried human pituitary glands. The major portion of growth hormone, follicle-stimulating hormone, luteinizing hormone and thyroid-stimulating hormone activities are each obtained in a separate fraction. Although the yields of the individual hormones are similar to those obtained by other investigators, the present procedure is simpler and a cleaner separation of the hormones has been achieved. Methods for further purification of each fraction are indicated including preparation of luteinizing hormone of very high potency (equivalent to 5.3mg. of standard NIH-LH-S3/mg.).
摘要
已开发出一种从丙酮干燥的人垂体中分离和部分纯化四种蛋白质激素的方法。生长激素、促卵泡激素、促黄体生成素和促甲状腺激素活性的主要部分分别在一个单独的组分中获得。虽然各激素的产量与其他研究者获得的产量相似，但本方法更简单，且已实现了激素的更纯净分离。文中指出了进一步纯化每个组分的方法，包括制备高效能的促黄体生成素（相当于每毫克5.3毫克标准NIH-LH-S3）。
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