源自皮肤纤维肉瘤的结缔组织大分子的生化特性

Biochemical characterization of connective tissue macromolecules derived from cutaneous fibrosarcoma.

作者信息

Matsubayashi S, Shinkai H, Sano S

出版信息

Arch Dermatol Res (1975). 1977 Dec 12;260(2):93-102. doi: 10.1007/BF00561114.

Abstract

Tumor tissue of cutaneous fibrosarcoma was solubilized with salt, acetic acid and salt-extracted after pepsin treatment. Type III collagen was observed in neutral soluble collagen, 1.5 M and 2.4 M NaCl precipitated fractions after pepsin treatment. Type III collagen fraction precipitated with 1.5 M NaCl eluted in the alpha2 region when chromatographed on CM-cellulose without reduction, while the type III collagen partially purified with 1.5 M NaCl eluted between the position of alpha1(I) and beta12 after reduction and alkylation. Analysis of amino acid showed the presence of cysteine and the high content of hydroxyproline following CM-chromatography of 1.5 M NaCl precipitated type III collagen fraction. Acidic glycosaminoglycans were composed of hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparan sulfate, and the first two were major components of fibrosarcoma.

摘要

皮肤纤维肉瘤的肿瘤组织经盐、乙酸溶解，并在胃蛋白酶处理后进行盐提取。在胃蛋白酶处理后的中性可溶性胶原蛋白、1.5M和2.4M氯化钠沉淀组分中观察到III型胶原蛋白。用1.5M氯化钠沉淀的III型胶原蛋白组分，在未还原的情况下在CM - 纤维素上进行色谱分析时，在α2区域洗脱，而用1.5M氯化钠部分纯化的III型胶原蛋白在还原和烷基化后在α1(I)和β12位置之间洗脱。氨基酸分析表明，在对1.5M氯化钠沉淀的III型胶原蛋白组分进行CM - 色谱分析后，存在半胱氨酸且羟脯氨酸含量较高。酸性糖胺聚糖由透明质酸、硫酸软骨素、硫酸皮肤素和硫酸乙酰肝素组成，前两者是纤维肉瘤的主要成分。