Boulton R W, Westaway E G
Arch Virol. 1977;55(3):201-8. doi: 10.1007/BF01319906.
Electrophoretic analyses showed that no RNase-sensitive RNA smaller than the genome was specified by the flavivirus Kunjin in infected Vero cells during the period of maximum RNA and protein synthesis. In contrast, RNA extracted from Sindbis virus-infected cells under similar conditions included the expected 42S RNA (equivalent to the genome) and the smaller 26S (interjacent) RNA. Treatment of the genome of both togaviruses with 12 M urea produced a reversible (possibly conformational) change; measurement of the molecular weights of the treated RNAs by co-electrophoresis with fully denatured ribosomal RNA markers in SDS-polyacrylamide gels yielded a value of 2.1 X 10(6) if 8 M urea was incorporated in the gels and 4.2 X 10(6) if urea was omitted from the gels. These results indicate that flavivirus messenger RNA is represented solely by the intact genome of m.wt. 4.2 X 10(6).
电泳分析表明，在最大RNA和蛋白质合成期间，感染Vero细胞的黄病毒库京病毒未产生小于基因组的对核糖核酸酶敏感的RNA。相比之下，在类似条件下从辛德毕斯病毒感染的细胞中提取的RNA包括预期的42S RNA（相当于基因组）和较小的26S（中间）RNA。用12M尿素处理这两种披膜病毒的基因组会产生可逆（可能是构象）变化；在SDS-聚丙烯酰胺凝胶中与完全变性的核糖体RNA标志物共电泳来测量处理后RNA的分子量，如果凝胶中加入8M尿素，得到的值为2.1×10⁶，如果凝胶中不加入尿素，则为4.2×10⁶。这些结果表明，黄病毒信使RNA仅由分子量为4.2×10⁶的完整基因组代表。
