Ozden S, Poirier B
Arch Virol. 1985;85(1-2):129-37. doi: 10.1007/BF01317012.
Dengue 2 virus polypeptide synthesis was investigated in BHK21 cells. Nine or ten virus induced polypeptides were identified, three of which are glycoproteins as demonstrated by tunicamycin treatment of infected cells. We performed pulse chase experiments, experiments with amino-acid analogs, protease inhibitors or pactamycin treatment of infected cells, to determine whether or not large polypeptide processing occurs. In some of these experiments a large polypeptide (P 130) was immunoprecipitated by an anti-dengue 2 serum. We observed a transfer of label between small molecular weight polypeptides which might be the result of restricted proteolytic cleavage.
在BHK21细胞中研究了登革2型病毒的多肽合成。鉴定出九种或十种病毒诱导的多肽，其中三种为糖蛋白，这是通过用衣霉素处理感染细胞所证实的。我们进行了脉冲追踪实验、用氨基酸类似物、蛋白酶抑制剂或用 pactamycin 处理感染细胞的实验，以确定是否发生大的多肽加工过程。在其中一些实验中，一种大的多肽（P130）被抗登革2血清免疫沉淀。我们观察到小分子多肽之间的标记转移，这可能是有限的蛋白水解切割的结果。
