Tanino T, Saito M, Egawa K
Int J Cancer. 1976 Jun 15;17(6):736-41. doi: 10.1002/ijc.2910170608.
Cell-line specific cell surface components were demonstrated on cells of various hypotetraploid ascites cell lines, but not on hyperdiploid cells derived from a mammary carcinoma induced by mammary tumor virus (MTV) in syngeneic C3H/He mice. These substances caused cell-line-specific transplantation antigenicity and were identified, together with MTV-associated substances and tumor-associatied embryonic materials, as being the binding sites for tumor cell agglutinating factors found in sera from tumor-bearing and regressor animals. Particles containing the cell line-specific substances were released from these cells by hypnotic treatment. They could be purified as a single peak in a sucrose density gradient. They were, however, not dissociated from the MTV-associated substances. Absorption studies of agglutination activity were extensively used for demonstration of specificity.
在各种亚四倍体腹水细胞系的细胞上证实了细胞系特异性细胞表面成分，但在同基因C3H/He小鼠中由乳腺肿瘤病毒（MTV）诱导的乳腺癌衍生的超二倍体细胞上未发现。这些物质引起细胞系特异性移植抗原性，并与MTV相关物质和肿瘤相关胚胎物质一起被鉴定为荷瘤动物和消退动物血清中发现的肿瘤细胞凝集因子的结合位点。通过催眠处理从这些细胞中释放出含有细胞系特异性物质的颗粒。它们可以在蔗糖密度梯度中作为单一峰进行纯化。然而，它们并未与MTV相关物质解离。凝集活性的吸收研究被广泛用于特异性的证明。
