In vitro effects of Cytochalasin B on TA3 tumor cells.

We studied the effect of Cytochalasin B (CB) on two lines of the ascites tumor TA3 by microcinematography and scanning electron microscopy. CB induces a dose dependent and reversible cell paralysis. One microgram CB/ml causes a pronounced but incomplete paralysis. The cells retain their general shape but develop numerous blebs. Ten microgram CB/ml causes a complete cell paralysis and a retraction of pseudopods and microvilli. When CB is removed, the cells rapidly regain their original form and motility. The recovery process starts within minutes after CB removal and is almost completed in 15 min.