Gipson I K, Keezer L
Invest Ophthalmol Vis Sci. 1982 May;22(5):643-50.
To determine the effects of cytochalasins and colchicine on the ultrastructure of migrating corneal epithelium of the rat, abraded corneas healing in organ culture were cultured for 30 min in the presence of these cytoskeletal-perturbing drugs. Transmission and scanning electron microscopy of these corneas indicate the following. (1) Cytochalasins B (1 microgram/ml of medium) and D (0.1 microgram/ml of medium) caused drastic alteration in structure of cells of the leading edge only. Stratified layers of cells behind the leading edge were unaffected. This observation may indicate that it is the cells of the leading edge which have a rapid turnover of actin filament formation during epithelial sheet movement. (2) Both cytochalasins caused surface blebs or zeiotic processes to form on cells of the leading edge. The shape of the processes caused by the cytochalasins differed, however. Actin filaments accumulated in the cytoplasm under the zeiotic processes. (3) Colchicine had no effect on the ultrastructure of migrating epithelium.
为了确定细胞松弛素和秋水仙碱对大鼠角膜上皮迁移超微结构的影响，将在器官培养中愈合的擦伤角膜在这些细胞骨架干扰药物存在的情况下培养30分钟。这些角膜的透射电子显微镜和扫描电子显微镜观察结果如下：（1）细胞松弛素B（培养基中1微克/毫升）和D（培养基中0.1微克/毫升）仅导致前缘细胞结构发生剧烈改变。前缘后面的分层细胞层未受影响。这一观察结果可能表明，在上皮片层移动过程中，前缘细胞的肌动蛋白丝形成周转迅速。（2）两种细胞松弛素均导致前缘细胞表面形成泡状突起或病态变化。然而，细胞松弛素引起的突起形状不同。在病态变化下方的细胞质中积累了肌动蛋白丝。（3）秋水仙碱对迁移上皮的超微结构没有影响。
