• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

糖基酶与萘基底物的定位

Localization of glycoidases with naphthyl substrates.

作者信息

Gossrau R

出版信息

Histochem J. 1976 May;8(3):271-82. doi: 10.1007/BF01003816.

DOI:10.1007/BF01003816
PMID:59719
Abstract

Unsubstituted naphthyl substrates were found to be superior to substituted naphthyl, indolyl and hydroxyquinoline substrates for the histochemical demonstration of alpha-mannosidase, alpha-galactosidase, hetero-beta-glycosidase, glucoamylase and sucraseisomaltase, equivalent for beta-N-acetylglucosaminidase and lactase-beta-glucosidase, and inferior for beta-glucuronidase and acid beta-galatosidase. Aldehyde fixation is necessary for the localization of lysosomal glycosidases with naphthyl substrates. 1-naphthyl substrates are suitable for the detection of acid glycosidases in lysosomes and hetero-beta-glysocidase in the cytoplasm of animal cells, and 2-naphthyl substrates can be employed for the demonstration of microvillous glycosidases and for the evaluation of the total activity of soluble glycosidases with semipermeable membranes. When naphthyl substrates are used coupling should be carried out simultaneously and hexazotized pararosaniline is the coupling reagent of choice.

摘要

在α-甘露糖苷酶、α-半乳糖苷酶、杂β-糖苷酶、葡糖淀粉酶和蔗糖酶-异麦芽糖酶的组织化学显示中，未取代的萘基底物被发现优于取代的萘基、吲哚基和羟基喹啉底物；对β-N-乙酰葡糖胺酶和乳糖酶-β-葡糖苷酶而言相当；对β-葡糖醛酸酶和酸性β-半乳糖苷酶而言则较差。用萘基底物进行溶酶体糖苷酶定位时，醛固定是必要的。1-萘基底物适用于检测动物细胞溶酶体中的酸性糖苷酶和细胞质中的杂β-糖苷酶，2-萘基底物可用于显示微绒毛糖苷酶以及用半透膜评估可溶性糖苷酶的总活性。使用萘基底物时，偶联应同时进行，六重氮对苯二胺是首选的偶联试剂。

相似文献

1
Localization of glycoidases with naphthyl substrates.糖基酶与萘基底物的定位
Histochem J. 1976 May;8(3):271-82. doi: 10.1007/BF01003816.
2
[Histochemical and biochemical investigation of alpha-glucosidases by means of 2-naphthyl-alpha-D-glucoside (author's transl)].用2-萘基-α-D-葡萄糖苷对α-葡萄糖苷酶进行组织化学和生物化学研究（作者译）
Histochemistry. 1976 Oct 29;49(3):193-211. doi: 10.1007/BF00492375.
3
[Azoindoxyl methods for the investigation of hydrolases. IV. Suitability of various diazonium salts (author's transl)].[用于水解酶研究的偶氮吲哚酚方法。IV. 各种重氮盐的适用性（作者译）]
Histochemistry. 1978 Sep 28;57(4):323-42. doi: 10.1007/BF00492668.
4
Suitability of the azocoupling reaction with 1-naphthyl-beta-D-glucoside for the histochemical demonstration of lactase (lactase-beta-glucosidase complex) in human enterobiopsies.1-萘基-β-D-葡萄糖苷偶氮偶联反应用于人体肠活检中乳糖酶（乳糖酶-β-葡萄糖苷酶复合物）组织化学显示的适用性。
Histochemistry. 1975 Jun 9;43(4):349-53. doi: 10.1007/BF00490193.
5
Distribution of acid phosphatase, beta-glucuronidase, n-acetyl-beta-d-glucosaminidase and beta-galactosidase in cornea of albino rabbit.白化兔角膜中酸性磷酸酶、β-葡萄糖醛酸酶、N-乙酰-β-D-氨基葡萄糖苷酶和β-半乳糖苷酶的分布
Histochemistry. 1975 Sep 29;44(4):337-43. doi: 10.1007/BF00490370.
6
Biochemistry of the multiple forms of glycosidases in plants.植物中多种形式糖苷酶的生物化学
Adv Enzymol Relat Areas Mol Biol. 1984;56:141-249. doi: 10.1002/9780470123027.ch3.
7
Demonstration of "hetero-beta-galactosidase" "in situ".“异位β-半乳糖苷酶”的原位显示
Acta Univ Carol Med Monogr. 1977(79 Pt 3):99-105.
8
[Postnatal development of the epithelium of the small intestine of guinea-pigs (author's transl)].豚鼠小肠上皮的产后发育（作者译）
Prog Histochem Cytochem. 1978;11(2):1-67.
9
[Azoindoxyl methods for the histochemical investigation of hydrolases. I. Lactase (lactase-beta-glucosidase complex) (author's transl)].用于水解酶组织化学研究的偶氮吲哚酚法。I. 乳糖酶（乳糖酶-β-葡萄糖苷酶复合物）（作者译）
Histochemistry. 1976 Aug 12;48(2):111-9. doi: 10.1007/BF00494549.
10
[Histochemical demonstration of beta-glucuronidase, alpha-mannosidase and alpha-galactosidase using 1-naphthyl glycosides (author's transl)].使用1-萘基糖苷对β-葡萄糖醛酸酶、α-甘露糖苷酶和α-半乳糖苷酶进行组织化学显示（作者译）
Histochemie. 1973;36(4):367-81. doi: 10.1007/BF00305715.

引用本文的文献

1
Enzymatic activity and substrate specificity of recombinant tomato beta-galactosidases 4 and 5.重组番茄β-半乳糖苷酶4和5的酶活性及底物特异性
Planta. 2009 Jan;229(2):447-56. doi: 10.1007/s00425-008-0842-x. Epub 2008 Nov 6.
2
A family of at least seven beta-galactosidase genes is expressed during tomato fruit development.在番茄果实发育过程中，一个至少由七个β-半乳糖苷酶基因组成的家族得以表达。
Plant Physiol. 2000 Jul;123(3):1173-83. doi: 10.1104/pp.123.3.1173.
3
Quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method.

本文引用的文献

1
The colorimetric estimation of beta-d-glucosidase.β-D-葡萄糖苷酶的比色测定法
J Biol Chem. 1952 Apr;195(2):607-14.
2
The colorimetric estimation and histochemical demonstration of beta-d-galactosidase.β - D - 半乳糖苷酶的比色测定及组织化学显示
J Biol Chem. 1952 Mar;195(1):239-49.
3
The localization of N-acetyl-beta-glucosaminidase in tissues.N-乙酰-β-葡萄糖苷酶在组织中的定位。
甘蓝型油菜正常和酶缺陷（gal）花粉中β-半乳糖苷酶的定量细胞化学：靛蓝生成法的应用
Histochem J. 1984 Dec;16(12):1273-96. doi: 10.1007/BF01003726.
4
[Distribution of some hydrolases in the rat kidney (author's transl)].大鼠肾脏中某些水解酶的分布（作者译）
Histochemistry. 1981;71(3):463-80. doi: 10.1007/BF00495886.
5
[Acid hydrolases in the epididymal epithelium of rats after castration and cryptorchidism (author's transl)].去势和隐睾大鼠附睾上皮中的酸性水解酶（作者译）
Histochemistry. 1981;71(3):361-404. doi: 10.1007/BF00495882.
6
Single-villus analysis of disaccharidase expression by different regions of the mouse intestine.对小鼠肠道不同区域二糖酶表达进行单绒毛分析。
J Physiol. 1988 Jul;401:533-45. doi: 10.1113/jphysiol.1988.sp017177.
7
[Histochemical and biochemical investigation of alpha-glucosidases by means of 2-naphthyl-alpha-D-glucoside (author's transl)].用2-萘基-α-D-葡萄糖苷对α-葡萄糖苷酶进行组织化学和生物化学研究（作者译）
Histochemistry. 1976 Oct 29;49(3):193-211. doi: 10.1007/BF00492375.
8
[Suitability of naphthyl-alpha-L-fucosides for the investigation of alpha-L-fucosidases (author's transl)].萘基-α-L-岩藻糖苷用于α-L-岩藻糖苷酶研究的适用性（作者译）
Histochemistry. 1977 Jun 8;52(3):259-71. doi: 10.1007/BF00495861.
9
[Azoindoxyl methods for the investigation of hydrolases. IV. Suitability of various diazonium salts (author's transl)].[用于水解酶研究的偶氮吲哚酚方法。IV. 各种重氮盐的适用性（作者译）]
Histochemistry. 1978 Sep 28;57(4):323-42. doi: 10.1007/BF00492668.
10
[Peptidases. I. Histochemical investigations with 2-naphthylamides and hexazonium-p-rosanilin (author's transl)].[肽酶。I. 用2-萘酰胺和六偶氮对苯二胺（作者译）进行的组织化学研究]
Histochemistry. 1977 Dec 28;54(4):311-30. doi: 10.1007/BF00508274.
J Histochem Cytochem. 1961 May;9:242-50. doi: 10.1177/9.3.242.
4
Histochemical localization of beta-glucuronidase and N-acetyl-beta-glucosaminidase.β-葡萄糖醛酸酶和N-乙酰-β-氨基葡萄糖苷酶的组织化学定位
J Histochem Cytochem. 1961 Jan;9:105-6. doi: 10.1177/9.1.105.
5
The histochemical demonstration of alpha-D-glucosidase in mammalian tissues.哺乳动物组织中α-D-葡萄糖苷酶的组织化学显示
J Histochem Cytochem. 1960 Jul;8:268-72. doi: 10.1177/8.4.268.
6
Significance of coupling rate in histochemical azo dye methods for enzymes.组织化学偶氮染料法中酶偶联率的意义
J Histochem Cytochem. 1955 May;3(3):203-11. doi: 10.1177/3.3.203.
7
HISTOCHEMICAL DEMONSTRATION OF N-ACETYL-BETA-GLUCOSAMINIDASE EMPLOYING NAPHTHOL AS-BI N-ACETYL-BETA -GLUCOSAMINIDE AS SUBSTRATE.以萘酚AS - BI N - 乙酰 - β - 氨基葡萄糖苷为底物的N - 乙酰 - β - 氨基葡萄糖苷酶的组织化学显示
J Histochem Cytochem. 1965 May-Jun;13:355-60. doi: 10.1177/13.5.355.
8
RAT INTESTINAL 6-BROMO-2-NAPHTHYL GLYCOSIDASE AND DISACCHARIDASE ACTIVITIES. I. ENZYMIC PROPERTIES AND DISTRIBUTION IN THE DIGESTIVE TRACT OF CONVENTIONAL AND GERM-FREE ANIMALS.大鼠肠道6-溴-2-萘基糖苷酶和双糖酶活性。I. 常规动物和无菌动物消化道中的酶学特性及分布
Arch Biochem Biophys. 1965 Jan;109:150-8. doi: 10.1016/0003-9861(65)90300-0.
9
TISSUE ENZYME FIXATION STUDIES. 1. THE EFFECTS OF ALDEHYDE FIXATION ON BETA-GLUCURONIDASE, BETA-GALACTOSIDASE, N-ACETYL-BETA-GLUCOSAMINIDASE, AND BETA-GLUCOSIDASE IN TISSUE BLOCKS.组织酶固定研究。1. 醛类固定对组织块中β-葡萄糖醛酸酶、β-半乳糖苷酶、N-乙酰-β-氨基葡萄糖苷酶和β-葡萄糖苷酶的影响
Lab Invest. 1964 Sep;13:1038-50.
10
THE CYTOLOGIC DEMONSTRATION OF BETA-GLUCURONIDASE EMPLOYING NAPHTHOL AS-BI GLUCURONIDE AND HEXAZONIUM PARAROSANILIN; A PRELIMINARY REPORT.使用萘酚 AS - BI 葡糖苷酸和副品红六偶氮化物进行β - 葡糖醛酸酶的细胞化学显示：初步报告。
J Histochem Cytochem. 1964 Apr;12:293-7. doi: 10.1177/12.4.293.