Wolfe S L, Hewitt G M
J Cell Biol. 1966 Oct;31(1):31-42. doi: 10.1083/jcb.31.1.31.
Meiotic chromosomes were isolated from male Oncopeltus fasciatus by dissecting the testes under insect Ringer's solution and spreading the living cells on the Langmuir trough. After being dried by the critical point method, preparations were examined under the electron microscope. Chromosomes at all stages of prophase prove to be multistranded. A significant increase in the number of parallel 250 A fibers in the chromosomes occurs between zygotene and diakinesis. Parallel folding, rather than true multistrandedness, is interpreted as the mechanism responsible for this observed increase in multistrandedness. It has not been possible to determine whether the multistrandedness observed at leptotene represents true multistrandedness or is the result of parallel folding. Apparent multistrandedness is lost at metaphase when the 250 A fibers of the chromosomes become coiled more tightly. In preparations isolated by these methods, no structures other than the 250 A chromosome fibers are visible in the chromomeres, which appear as regionally coiled or folded areas of the fibers along the arm of the chromosome.
通过在昆虫林格氏液中解剖睾丸并将活细胞铺展在朗缪尔槽上，从雄性大斑芫菁中分离出减数分裂染色体。经临界点干燥法干燥后，在电子显微镜下检查标本。前期各阶段的染色体均为多股结构。在偶线期到终变期之间，染色体中平行的250埃纤维数量显著增加。平行折叠而非真正的多股结构被认为是导致观察到的多股结构增加的机制。目前尚无法确定细线期观察到的多股结构是真正的多股结构还是平行折叠的结果。当染色体的250埃纤维紧密盘绕时，中期明显的多股结构消失。在通过这些方法分离的标本中，除了250埃的染色体纤维外，在染色粒中没有可见的其他结构，染色粒表现为沿着染色体臂的纤维局部盘绕或折叠区域。
