大鼠肝脏线粒体对亚砷酸盐的能量依赖性摄取。
Energy-dependent uptake of arsenite by rat liver mitochondria.
作者信息
Harris E J, Achenjang F M
出版信息
Biochem J. 1977 Oct 15;168(1):129-32. doi: 10.1042/bj1680129.
Abstract
Uptake of arsenite by rat liver mitochondria is energy-dependent, as shown by comparing values without and with either uncoupling agent or respiratory inhibitor present. The uptake is inhibited by mersalyl and N-ethylmaleimide, which can be used as 'stopping' agents to obtain uptake kinetics. At 20 degrees C the process is nearly complete in 1 min. The relation between the quantity in the energized mitochondria and the applied concentration corresponds to at least two different modes of binding of the arsenite. Competition occurs between arsenite and other anions (for example, phosphate) for intramitochondrial accumulation.
摘要
大鼠肝线粒体对亚砷酸盐的摄取是能量依赖性的，这一点可通过比较有无解偶联剂或呼吸抑制剂存在时的摄取值来证明。摄取受到汞撒利和N - 乙基马来酰胺的抑制，它们可用作“终止”剂以获得摄取动力学。在20摄氏度时，该过程在1分钟内几乎完成。线粒体中活化状态下的量与施加浓度之间的关系至少对应于亚砷酸盐的两种不同结合模式。亚砷酸盐与其他阴离子（例如磷酸盐）之间存在线粒体内部积累的竞争。
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Biochemistry. 1962 Mar;1:276-81. doi: 10.1021/bi00908a014.
2
3
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