Lee W H, McGrath P P, Carter P H, Eide E L
Can J Microbiol. 1977 Dec;23(12):1714-22. doi: 10.1139/m77-247.
Many types of Yersinia enterocolitica have been isolated from animal, environmental, food, and human sources but their public health significance remains uncertain. Seventy two strains of Y. enterocolitica were tested for their abilities to invade HeLa cells. The typical clinical strains invade HeLa cells like the other species of invasive pathogens. This characteristic remains even in old stock cultures and can be temperature-sensitive like the motility characteristic. With the use of electron micrographs it was demonstrated that the bacteria were truly intracellular and not merely adhering to the HeLa cell membrane. The esculin-and salicin-positive typical clinical strains did not invade HeLa cells. None of 34 food and water isolates were invasive by this test. The negative Y. enterocolitica strains did not adhere to the cells and cause ambiguous results. The HeLa cell test is simple, inexpensive, rapid, and should prove useful marker for screening the Y. enterocolitica isolates.
已从动物、环境、食物和人类来源分离出多种小肠结肠炎耶尔森菌，但它们对公共卫生的重要性仍不确定。对72株小肠结肠炎耶尔森菌进行了侵袭HeLa细胞能力的测试。典型的临床菌株像其他侵袭性病原体物种一样侵袭HeLa细胞。即使在陈旧的菌种培养物中，这一特性仍然存在，并且可能像运动特性一样对温度敏感。通过电子显微镜照片证明，细菌确实存在于细胞内，而不仅仅是附着在HeLa细胞膜上。七叶苷和水杨苷阳性的典型临床菌株不侵袭HeLa细胞。通过该测试，34株食物和水分离株均无侵袭性。阴性小肠结肠炎耶尔森菌菌株不黏附细胞，结果不明确。HeLa细胞试验简单、廉价、快速，应可证明是筛选小肠结肠炎耶尔森菌分离株的有用标志物。
