Richmond V, Miller J L, Stegall P J, Smith K H
Clin Orthop Relat Res. 1977 Jul-Aug(126):292-301.
Experimental dysbaric osteonecrosis associated with inadequate decompression has been induced in mature female swine. The bone collagen from the radiologically defined lesion in the femur has been analyzed. The total collagen contents were the same for the epiphyseal (F-1), the metaphyseal (F-2), and the diaphyseal (F-3) regions. Soluble bone collagen content obtained by sequential extraction of the samples with acetic acid urea, guanidine, lithium chloride and potassium thiocyanate, decreased by approximately 20% in going from the F-1 to the F-2 to the F-3 regions for both experimental and control samples. The amino acid compositions and acrylamide gel electrophoresis patterns were the same for control and experimental samples. Spectral changes with the MBTH reaction were different for control and experimental collagen samples from all bone regions. The spectral shift and pattern changes which occurred from pH 4 to pH 1 suggested a higher average content of unsaturation in the collagen associated aldehyde groups in the experimental than in the control samples. These findings suggest an enhanced ability to remodel collagen which would be consistent with the histological observation of increased derangement of collagen fibers in the experimental bone samples.
在成年雌性猪身上诱发了与减压不充分相关的实验性减压病性骨坏死。对股骨中经放射学界定的病变部位的骨胶原进行了分析。骨骺（F-1）、干骺端（F-2）和骨干（F-3）区域的总胶原含量相同。通过用醋酸尿素、胍、氯化锂和硫氰酸钾对样品进行顺序提取得到的可溶性骨胶原含量，在实验样品和对照样品中，从F-1区域到F-2区域再到F-3区域均下降了约20%。对照样品和实验样品的氨基酸组成及丙烯酰胺凝胶电泳图谱相同。来自所有骨区域的对照胶原样品和实验胶原样品与MBTH反应的光谱变化不同。从pH 4到pH 1发生的光谱位移和图谱变化表明，实验样品中胶原相关醛基的平均不饱和含量高于对照样品。这些发现提示胶原重塑能力增强，这与实验性骨样品中胶原纤维排列紊乱增加的组织学观察结果一致。
