Gregory S P, Hilder V A, Maclean N
J Cell Sci. 1977 Dec;28:49-60. doi: 10.1242/jcs.28.1.49.
Nuclei isolated from Xenopus erythrocytes can be transcriptionally reactivated by exposure to certain cytoplasmic proteins. The types of RNA synthesized during this reactivation have been studied and compared with those present in, or synthesized by, isolated nuclei not so reactivated or in entire Xenopus erythrocytes. In all cases, the pattern of transcription indicates the synthesis of a broad range of low molecular weight RNAs. Competitive hybridization demonstrates that the reactivated nuclei synthesize some transcripts not normally produced by the isolated nuclei and we have shown that a proportion of these possess amino acid-accepting activity. The significance of these results is discussed in relation to the control of gene activity in these cells.
从非洲爪蟾红细胞中分离出的细胞核，通过暴露于某些细胞质蛋白可被转录重新激活。已对这种重新激活过程中合成的RNA类型进行了研究，并与未如此重新激活的分离细胞核或完整非洲爪蟾红细胞中存在的或合成的RNA进行了比较。在所有情况下，转录模式均表明合成了多种低分子量RNA。竞争性杂交表明，重新激活的细胞核合成了一些分离细胞核通常不产生的转录本，并且我们已经表明，这些转录本中有一部分具有氨基酸接受活性。结合这些细胞中基因活性的控制对这些结果的意义进行了讨论。
