Maclean N, Hilder V A
J Cell Sci. 1977 Apr;24:119-29. doi: 10.1242/jcs.24.1.119.
The effects of exposing nuclei isolated from Xenopus erythrocytes to cytoplasmic extracts prepared from various sources on their template activity are described. The cytoplasm of rat liver cells and the immature blood cells of Xenopus contain factors which stimulate RNA synthesis in these essentially inactive nuclei, whereas the cytoplasm of many other cells does not yield such factors. The active factors in rat liver cytoplasmic extracts appear to be proteins of molecular weight about 40000 Daltons. The implications of these results for genetic control in eukaryotes are discussed.
本文描述了将非洲爪蟾红细胞分离出的细胞核暴露于从各种来源制备的细胞质提取物中对其模板活性的影响。大鼠肝细胞的细胞质和非洲爪蟾未成熟血细胞中含有能刺激这些基本无活性细胞核中RNA合成的因子，而许多其他细胞的细胞质则不会产生此类因子。大鼠肝脏细胞质提取物中的活性因子似乎是分子量约为40000道尔顿的蛋白质。本文还讨论了这些结果对真核生物基因控制的意义。
