Graham-Pole J, Davie M, Willoughby M L
J Clin Pathol. 1977 Aug;30(8):758-62. doi: 10.1136/jcp.30.8.758.
Human granulocytes (PMNL) were successfully cryopreserved for up to 14 months. The PMNL (1-2 X 10(7)/ml) were stored in 2-ml ampoules in the gas phase of liquid nitrogen at a temperature between -160 degrees C and -196 degrees C using dimethylsulphoxide (DMSO 10%) as cryoprotectant. Morphology and phagocytic and bactericidal capacity were best preserved by adding fetal calf serum to the freezing mixture, by using an interrupted cooling process, by washing the thawed PMNL in fresh freeze-dried plasma, and centrifuging at 600 g for no more than two minutes. Careful post-thaw handling of the cells was an important factor in preserving function. These preliminary studies indicate that useful numbers of PMNL can be recovered in a functional state after storage for long periods in liquid nitrogen.
人类粒细胞（多形核白细胞，PMNL）成功冷冻保存长达14个月。将PMNL（1 - 2×10⁷/ml）以10%二甲基亚砜（DMSO）作为冷冻保护剂，储存在2毫升安瓿瓶中，置于液氮气相中，温度在-160℃至-196℃之间。通过在冷冻混合物中添加胎牛血清、采用间断冷却过程、用新鲜冻干血浆洗涤解冻后的PMNL并以600g离心不超过两分钟，可最佳地保存其形态以及吞噬和杀菌能力。细胞解冻后的小心处理是保存功能的一个重要因素。这些初步研究表明，在液氮中长时间储存后，能回收功能状态良好的大量PMNL。
