药物致敏的实验研究。II. 抗乙酰水杨酸抗体的产生及其被动血凝检测
Experimental studies on drug sensitization. II. Production of antiacetylsalicylyl antibodies and their detection by passive hemagglutination.
作者信息
Cîrstea M, Suhaciu G
出版信息
Physiologie. 1976 Apr-Jun;13(2):135-40.
Antiacetylsalicylyl antibodies were produced in rabbits and guinea-pigs by innoculation of acetylsalicylic acid incorporated in Freund adjuvant. These antibodies were readily detected by a passive hemagglutination test using rabbit erythrocytes incubated with acetylsalicylic acid at alkaline pH. Acetylsalicylyl conjugates with human gammaglobulin, bovine gammaglobulin and rabbit gammaglobulin were also prepared by incubating the proteins with acetylsalicylic acid at alkaline pH. In parallel experiments, salicylic acid did not induce formation of specific antibodies capable of reacting with acetylsalicylic or salicylic acid-treated erythrocytes.
通过在弗氏佐剂中接种乙酰水杨酸，在兔和豚鼠体内产生了抗乙酰水杨酰抗体。使用在碱性pH下与乙酰水杨酸孵育的兔红细胞，通过被动血凝试验可轻松检测到这些抗体。通过在碱性pH下将蛋白质与乙酰水杨酸孵育，还制备了与人γ球蛋白、牛γ球蛋白和兔γ球蛋白的乙酰水杨酰缀合物。在平行实验中，水杨酸不会诱导形成能够与乙酰水杨酸或经水杨酸处理的红细胞发生反应的特异性抗体。
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