HLA - D 决定簇分型。使用纯合刺激细胞和致敏培养物的分型结果比较。
Typing for HLA-D determinants. comparison of typing results using homozygous stimulating cells and primed cultures.
作者信息
Hirschberg H, Kaakinen A, Thorsby E
出版信息
Tissue Antigens. 1976 Apr;7(4):213-9. doi: 10.1111/j.1399-0039.1976.tb01057.x.
Abstract
Typing for HLA-D determinants, using primed lymphocyte typing (PLT), has been compared to the results obtained using conventional homozygous stimulating cell primary MLC tests. The HLA-Dwl, -Dw2 and -Dw3 specificities were studied. Preliminary results indicate that these two methods essentially type for the HLA-D determinants and that reproducible results can be obtained employing the PLT technique after 24 hours of incubation in the secondary cultures.
摘要
采用预处理淋巴细胞分型法（PLT）对HLA - D决定簇进行分型，并与使用传统纯合刺激细胞初次混合淋巴细胞培养试验所获得的结果进行了比较。对HLA - Dw1、- Dw2和- Dw3特异性进行了研究。初步结果表明，这两种方法本质上都是对HLA - D决定簇进行分型，并且在二次培养中孵育24小时后，采用PLT技术可获得可重复的结果。
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