Romanova A K, Nozhevnikova A N, Leont'ev I G, Alekseeva S A
Mikrobiologiia. 1977 Sep-Oct;46(5):885-9.
Assimilation products of 14C-bicarbonate and carbon-14C oxide were studied in two carboxydobacteria Seliberia carboxydohydrogena and Achromobacter carboxydus which differed in their ability for chemolithoautrophous growth in the presence of hydrogen. The dynamics and composition of labeled products formed upon assimilation of 14C-bicarbonate in the presence of unlabeled carbon oxide by the two organisms, the composition of products formed upon assimilation of 14CO by suspensions of S. carboxydohydrogena Z-1062 during 5 minutes, and the dynamics and composition of labeled assimilates of A. carboxydus Z-1171 after incubation in the presence of 14CO, were found to be consistent with those expected in the action of the reductive pentose phosphate Calvin cycle. The similarity of products formed upon assimilation of 14CO2 and 14CO suggests that CO is first oxidized to CO2, and only is assimilated.
研究了两种羧基细菌——嗜氢羧基单胞菌（Seliberia carboxydohydrogena）和羧基无色杆菌（Achromobacter carboxydus）中¹⁴C - 碳酸氢盐和¹⁴C - 氧化碳的同化产物，这两种细菌在氢气存在下进行化能自养生长的能力有所不同。在未标记的二氧化碳存在下，两种生物体同化¹⁴C - 碳酸氢盐时形成的标记产物的动力学和组成、嗜氢羧基单胞菌Z - 1062悬浮液在5分钟内同化¹⁴CO时形成的产物组成，以及羧基无色杆菌Z - 1171在¹⁴CO存在下孵育后标记同化产物的动力学和组成，均与还原戊糖磷酸卡尔文循环作用中预期的情况一致。¹⁴CO₂和¹⁴CO同化时形成的产物相似性表明，CO首先被氧化为CO₂，然后才被同化。
