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[以改变的气体混合物组成培养羧基氢气自养菌]

[Growth of Seliberia carboxydohydrogena carboxy bacteria with an altered composition of the gas mixture].

作者信息

Volova T G, Stasishina G N, Kasaeva G E

出版信息

Mikrobiologiia. 1983 Jul-Aug;52(4):533-7.

PMID:6417461
Abstract

The growth and gas exchange of Seliberia carboxydohydrogena Z-1062 were studied in the regime of turbidostat when the conditions of gaseous nutrition were changed: a decrease in hydrogen concentration and an increase in carbon monoxide concentration, growth on two carbon sources (CO+CO2) and on two energy sources (H2+CO). The inhibition of the bacterial growth by CO was expressed in a decrease of the specific growth rate and in the reduced effectiveness of using a gaseous substrate. When the concentration of carbon monoxide was elevated from 0 to 40% and that of hydrogen was reduced from 80 to 40%, the specific growth rate of the cells was decreased from 0.4 to 0.04 h-1; here, the economic coefficient in terms of hydrogen fell from 3.6 to 0.62 g/g. The CO-oxidizing system of the bacterium was shown to be resistant. The rate of CO oxidation by the culture was from 0.6 to 0.8 L/h per 1 g of the synthesized biomass at the following concentration of gases in the medium (%); H2, 80-40; CO2, 5; O2, 15; CO, 10-40. The rate of CO oxidation by the culture rose when hydrogen concentration was decreased and CO concentration was increased.

摘要

在恒浊器培养条件下,研究了一氧化碳嗜氢菌Z-1062的生长及气体交换情况,其中气态营养条件发生了变化:氢气浓度降低,一氧化碳浓度升高,在两种碳源(CO+CO2)和两种能源(H2+CO)上生长。CO对细菌生长的抑制表现为比生长速率降低以及气态底物利用效率降低。当一氧化碳浓度从0升高至40%,氢气浓度从80%降低至40%时,细胞的比生长速率从0.4 h-1降至0.04 h-1;此时,以氢气计的经济系数从3.6 g/g降至0.62 g/g。结果表明该细菌的CO氧化系统具有抗性。在培养基中气体浓度如下(%)时,每1 g合成生物量的培养物对CO的氧化速率为0.6至0.8 L/h;H2,80 - 40;CO2,5;O2,15;CO,10 - 40。当氢气浓度降低且一氧化碳浓度升高时,培养物对CO的氧化速率上升。

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