Kvasnikov E I, Gavrilenko M N, Sumnevich V G, Stepaniuk V V, Eluseeva G S, Stognii I P
Mikrobiologiia. 1977 Sep-Oct;46(5):944-53.
A large number of bacterial strains assimilating chemical ethanol has been isolated using an original technique. Active growth of strains belonging to the genera Pseudomonas and, particularly, Acinetobacter was registered on mineral media containing ethanol. A mathematical model was constructed select a strain of Acinetobacter calcoaceticus K-9 during its continuous cultivation on media containing ethanol. The model makes it possible to determine conditions for producing a present amount of the biomass, the percentage of its yield, and the produc;iveness as a function of the dilution rate, temperature, and the concentration of ethanol and phosphoric acid in the medium. The main characteristics of the growth process in the studied factor space were established. The optimum conditions were calculated for growth of the strain with respect to each of the criteria. Under various conditions of bacterial growth, changes in the morphology and ultra-fine structure of the cells correlated with their physiological activity. The volume of the cells increased with the rate of dilution of the medium: the process can be described by a saturation curve. The presence of mesosomal structures is typical of the cells growing at low flow rates.
使用一种原始技术分离出了大量同化化学乙醇的细菌菌株。在含有乙醇的矿物培养基上记录到了假单胞菌属，特别是不动杆菌属菌株的活跃生长。构建了一个数学模型，用于在乙酸钙不动杆菌K-9菌株在含乙醇培养基上连续培养期间选择该菌株。该模型能够确定产生当前生物量的条件、其产量百分比以及作为稀释率、温度以及培养基中乙醇和磷酸浓度函数的生产率。确定了所研究因素空间中生长过程的主要特征。针对每个标准计算了菌株生长的最佳条件。在细菌生长的各种条件下，细胞形态和超微结构的变化与其生理活性相关。细胞体积随着培养基稀释率的增加而增大：该过程可用饱和曲线描述。中体结构的存在是低流速下生长细胞的典型特征。
