Alhadeff J A, Freeze H
Mol Cell Biochem. 1977 Nov 25;18(1):33-7. doi: 10.1007/BF00215277.
Human liver alpha-L-fucosidase was purified to apparent homogeneity and analyzed for carbohydrate content primarily by gas-liquid chromatography (glc). The enzyme is about 7% carbohydrate by weight and contains the following sugars (residues per 50, 000 molecular weight subunit): mannose (8.3), glucosamine (4.3) (presumably N-acetylated), sialic acid (1.6) and glucose (1.6). Galactose (0.8) and L-fucose (1.8) were also found but their presence may be due to artifacts of the purification procedure.
人肝α-L-岩藻糖苷酶被纯化至表观均一，并主要通过气液色谱法（glc）分析其碳水化合物含量。该酶按重量计约含7%的碳水化合物，含有以下糖类（每50,000分子量亚基中的残基）：甘露糖（8.3）、氨基葡萄糖（4.3）（推测为N-乙酰化）、唾液酸（1.6）和葡萄糖（1.6）。还发现了半乳糖（0.8）和L-岩藻糖（1.8），但其存在可能是纯化过程中的人为产物。
