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从耳软骨分离的软骨细胞单层培养和器官培养中的弹性纤维形成。

Elastic fiber formation in monolayer and organ cultures of chondrocytes isolated from auricular cartilage.

作者信息

Moskalewski S

出版信息

Am J Anat. 1976 Aug;146(4):443-8. doi: 10.1002/aja.1001460407.

DOI:10.1002/aja.1001460407
PMID:60053
Abstract

Chondrocytes were isolated from auricular cartilage of immature rabbits and maintained in monolayer or organ culture for 14 days. In both types of culture the chondrocytes formed conspicuous elastic fibers. In monolayer culture the fibers could be identified by orcein staining in the culture dish. Electron microscopy of organ cultures revealed the presence of two basic components of elastic fibers, i.e. microfibrils and elastin.

摘要

从幼年兔的耳廓软骨中分离出软骨细胞，并将其在单层培养或器官培养中维持14天。在这两种培养类型中，软骨细胞都形成了明显的弹性纤维。在单层培养中，这些纤维可通过在培养皿中用orcein染色来识别。器官培养的电子显微镜检查显示存在弹性纤维的两种基本成分，即微原纤维和弹性蛋白。

相似文献

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Elastic fiber formation in monolayer and organ cultures of chondrocytes isolated from auricular cartilage.从耳软骨分离的软骨细胞单层培养和器官培养中的弹性纤维形成。
Am J Anat. 1976 Aug;146(4):443-8. doi: 10.1002/aja.1001460407.
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Morphology, histochemistry, and differentiation of the cat's epiglottic cartilage: a supporting organ composed of elastic cartilage, fibrous cartilage, myxoid tissue, and fat tissue.猫会厌软骨的形态学、组织化学及分化：一个由弹性软骨、纤维软骨、黏液样组织和脂肪组织组成的支持器官。
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引用本文的文献

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Development of scaffold-free elastic cartilaginous constructs with structural similarities to auricular cartilage.具有与耳廓软骨结构相似性的无支架弹性软骨构建体的开发。
Tissue Eng Part A. 2014 Mar;20(5-6):1012-26. doi: 10.1089/ten.TEA.2013.0159. Epub 2014 Jan 21.
2
Influence of beta-aminoproprionitrile (BAPN) on cell growth and elastic fiber formation in cultures of auricular chondrocytes.β-氨基丙腈（BAPN）对耳软骨细胞培养中细胞生长和弹性纤维形成的影响。
Experientia. 1983 Oct 15;39(10):1147-8. doi: 10.1007/BF01943153.
3
Analysis of collagen types synthesized by rabbit ear cartilage chondrocytes in vivo and in vitro.
兔耳软骨软骨细胞在体内和体外合成的胶原类型分析。
Biochem J. 1984 Jul 1;221(1):189-96. doi: 10.1042/bj2210189.
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Reversible changes in nuclear and cell surface topography in cells exposed to collagenase and EDTA.暴露于胶原酶和乙二胺四乙酸（EDTA）的细胞中细胞核和细胞表面拓扑结构的可逆变化。
Cell Tissue Res. 1981;220(1):51-60. doi: 10.1007/BF00209965.
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Influence of proteolytic enzymes and calcium-binding agents on nuclear and cell surface topography.蛋白水解酶和钙结合剂对细胞核及细胞表面形貌的影响。
Cell Tissue Res. 1984;237(3):587-93. doi: 10.1007/BF00228443.
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In vitro elastic fiber formation by aggregated arotic cells of newborn rabbits.新生兔主动脉细胞聚集形成体外弹性纤维。
Anat Embryol (Berl). 1976 Dec 22;150(1):113-22. doi: 10.1007/BF00346290.
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Cell Tissue Res. 1977 May 31;180(3):341-56. doi: 10.1007/BF00227600.