Brissie R M, Spicer S S, Thompson N T
Anat Rec. 1975 Jan;181(1):83-94. doi: 10.1002/ar.1091810107.
Verhoeff's iron hematoxylin (VIH) followed by lead citrate (LC) applied to epoxy thin sections stained the dense component of elastic fibers heavily and the peripheral microfibrillar component lightly in guinea pig trachea and mouse testis fixed with a glutaraldehyde-osmium tetroxide sequence. This method stained large fimbriated fibers beneath tracheal epithelium, small fibers and stacked aggregates thereof in the deep lamina propria, cartilage and adventitia of the trachea and large stacked fibers in the fibroelastic band of the trachea. Fibers of the fetus differed from those of the adult, especially in the subepithelial elastic lamina of the trachea. Elastic fibers were intimately associated with fibroblasts and particularly slender fibroblast processes in tracheal stroma and with chondrocytes in tracheal cartilage. Fibroblasts associated with elastic fibers in the tracheal subepithelial lamina propria were often closely bordered by eosinophils, mast cells, or monocytes. Occasional mast cells extended slender processes around elastic fibers in the subepithelial lamina propria. In mouse testis and in many regions of the trachea, small elastic fibers were identified which were below the limits of resolution for the light microscope and were not apparent at the ultrastructural level in routinely stained thin sections.
将Verhoeff铁苏木精（VIH）接着用柠檬酸铅（LC）应用于经戊二醛 - 四氧化锇顺序固定的豚鼠气管和小鼠睾丸的环氧树脂薄切片上，弹性纤维的致密成分被重度染色，而外周微原纤维成分被轻度染色。该方法对气管上皮下方的大型有纤毛纤维、固有层深部的小型纤维及其堆叠聚集体、气管软骨和外膜以及气管纤维弹性带中的大型堆叠纤维进行了染色。胎儿的纤维与成人的不同，特别是在气管的上皮下弹性层。弹性纤维与气管基质中的成纤维细胞特别是细长的成纤维细胞突起以及气管软骨中的软骨细胞密切相关。气管上皮下固有层中与弹性纤维相关的成纤维细胞常常紧邻嗜酸性粒细胞、肥大细胞或单核细胞。偶尔有肥大细胞在固有层上皮下围绕弹性纤维伸出细长突起。在小鼠睾丸和气管的许多区域，发现了小型弹性纤维，其低于光学显微镜的分辨率极限，并且在常规染色的薄切片的超微结构水平上不明显。
