蝰亚科蛇类的胆汁酸以及来自极北蝰（Vipera berus (Linn.)）肝脏匀浆组分中C-23-羟基化胆汁酸的生物合成
Bile acids of snakes of the subfamily Viperinae and the biosynthesis of C-23-hydroxylated bile acids in liver homogenate fractions from the adder, Vipera berus (Linn.).
作者信息
Ikawa S, Tammar A R
出版信息
Biochem J. 1976 Feb 1;153(2):343-50. doi: 10.1042/bj1530343.
Abstract
- Analysis of bile salts of four snakes of the subfamily Viperinae showed that their bile acids consisted mainly of C-23-hydroxylated bile acids. 2. Incubations of 14C-labelled sodium cholate (3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholan-24-oate) and deoxycholate (3 alpha, 12 alpha-dihydroxy-5 beta-cholan-24-oate) with whole and fractionated adder liver homogenates were carried out in the presence of molecular oxygen and NADPH or an NADPH-generating system. The formation of C-23-hydroxylated bile acids, namely bitocholic acid (3 alpha, 12 alpha, 23xi-trihydroxy-5 beta-cholan-24-oic acid) and 3 alpha, 7 alpha, 12 alpha, 23 xi-tetrahydroxy-cholanic acid (3 alpha, 7 alpha, 12 alpha, 23 xi-tetrahydroxy-5 beta-cholan-24-oic acid), was observed mainly in the microsomal fraction and partly in the mitochondrial fraction. 3. Biosynthetic pathways of C-23-hydroxylated bile acids are discussed.
摘要
- 对蝰蛇亚科四种蛇的胆汁盐进行分析表明，它们的胆汁酸主要由C-23-羟基化胆汁酸组成。2. 在分子氧和NADPH或NADPH生成系统存在的情况下，用全蝰蛇肝脏匀浆和分级分离的蝰蛇肝脏匀浆对14C标记的胆酸钠（3α，7α，12α-三羟基-5β-胆烷-24-酸盐）和脱氧胆酸盐（3α，12α-二羟基-5β-胆烷-24-酸盐）进行孵育。C-23-羟基化胆汁酸，即二羟胆酸（3α，12α，23ξ-三羟基-5β-胆烷-24-酸）和3α，7α，12α，23ξ-四羟基胆酸（3α，7α，12α，23ξ-四羟基-5β-胆烷-24-酸）的形成主要在微粒体部分观察到，部分在线粒体部分观察到。3. 讨论了C-23-羟基化胆汁酸的生物合成途径。
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