粗制组织提取物中末端脱氧核苷酸转移酶检测常用方法的关键比较。
A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts.
作者信息
Coleman M S
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4305-12. doi: 10.1093/nar/4.12.4305.
Abstract
Terminal deoxynucleotidyl transferase (TdT) is a non-template directed DNA polymerase normally found in vertebrate thymus and bone marrow. Quantitative assay of TdT activity is being widely used as a tool in the differential diagnosis of acute leukemias in man. Clinical specimens of blood and bone marrow often contain 10(7) or fewer cells and require a specific and rapid assay for transferase which can be carried out in crude cell extracts. Commonly used assay methods do not meet these requirements, but can be easily modified to do so.
摘要
末端脱氧核苷酸转移酶（TdT）是一种无模板指导的DNA聚合酶，通常存在于脊椎动物的胸腺和骨髓中。TdT活性的定量测定作为一种工具，在人类急性白血病的鉴别诊断中得到了广泛应用。血液和骨髓的临床标本通常含有10⁷个或更少的细胞，需要一种能够在粗细胞提取物中进行的特异性快速转移酶测定方法。常用的测定方法不符合这些要求，但可以很容易地进行修改以满足这些要求。
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A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts.粗制组织提取物中末端脱氧核苷酸转移酶检测常用方法的关键比较。
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本文引用的文献
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THE ENZYMATIC CONDENSATION OF OLIGODEOXYRIBONUCLEOTIDES WITH POLYDEOXYRIBONUCLEOTIDES.寡脱氧核糖核苷酸与多脱氧核糖核苷酸的酶促缩合反应
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Enzymatic synthesis of oligodeoxynucleotides.寡脱氧核苷酸的酶促合成。
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Low molecular weight deoxyribonucleic acid polymerase in mammalian cells.哺乳动物细胞中的低分子量脱氧核糖核酸聚合酶。
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Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow.兔骨髓来源的低分子量脱氧核糖核酸聚合酶
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Doxynucleotide-polymerizing enzymes of calf thymus gland. IV. Inhibition of terminal deoxynucleotidyl transferase by metal ligands.小牛胸腺的脱氧核苷酸聚合酶。IV. 金属配体对末端脱氧核苷酸转移酶的抑制作用
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1041-8. doi: 10.1073/pnas.65.4.1041.
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Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme.来自小牛胸腺染色质的低分子量脱氧核糖核酸聚合酶。I. 纯酶的制备。
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