Luedecke L O, Harmon L G
Appl Microbiol. 1966 Sep;14(5):716-9. doi: 10.1128/am.14.5.716-719.1966.
Similar populations of Pseudomonas fragi were grown at 25 C for 20 hr or at 7 C for 7 days in milk containing 0, 10, and 20% fat; they were then heated at 48, 50, and 52 C in milk containing 0, 10, and 20% fat. After inoculation, the heating medium contained 2.1 x 10(6) to 6.9 x 10(6) organisms per milliliter. The P. fragi cells grown in skim milk had greater thermal resistance (D(52) = 3.0 to 3.1) than those grown in milk containing fat (D(52) = 1.9 to 2.5). The organisms grown at 7 C for 7 days in milk containing 10% fat were more resistant (D(52) = 3.0) than those grown in the same medium at 25 C for 20 hr (D(52) = 2.0). The presence of 0 to 20% milk fat in the heating medium had no apparent effect on the thermal resistance.
将弗氏假单胞菌的相似菌群体分别在含0%、10%和20%脂肪的牛奶中于25℃培养20小时或在7℃培养7天；然后在含0%、10%和20%脂肪的牛奶中于48℃、50℃和52℃加热。接种后，加热培养基中每毫升含有2.1×10⁶至6.9×10⁶个菌体。在脱脂牛奶中生长的弗氏假单胞菌细胞比在含脂肪牛奶中生长的细胞具有更高的耐热性（D₅₂ = 3.0至3.1）。在含10%脂肪的牛奶中于7℃培养7天的菌体比在相同培养基中于25℃培养20小时的菌体更具抗性（D₅₂ = 3.0）。加热培养基中0%至20%的乳脂肪对耐热性无明显影响。
