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荧光假单胞菌在添加不同脂质的冷生脱脂乳中的脂肪酶活性。

Lipase activity of Pseudomonas fluorescens in cold raw skim milk with different lipid supplements.

作者信息

Perez-Esteban J, SanJose C, Jaspe A

机构信息

Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

出版信息

Folia Microbiol (Praha). 1997;42(4):345-8. doi: 10.1007/BF02816947.

DOI:10.1007/BF02816947
PMID:9449781
Abstract

Different dairy-like fat fractions were added to raw skim milk in order to determine their effect on lipase production by Pseudomonas fluorescens growing on raw milk at 7 degrees C. Supplementation with preparations of fat globule membranes, mixtures of capric acid, glycerol monocaprinate, and glycerol dicaprinate, cholesterol, or lecithin at concentrations similar to those occurring in whole milk had only slight effects on lipase production, whereas supplementation with 0.9% glycerol trioleate decreased lipase activity in milk by 90%.

摘要

为了确定不同的类乳脂肪组分对荧光假单胞菌在7摄氏度生鲜牛奶中生长时产生脂肪酶的影响,将其添加到生鲜脱脂牛奶中。添加与全脂牛奶中浓度相似的脂肪球膜制剂、癸酸、单癸酸甘油酯、双癸酸甘油酯、胆固醇或卵磷脂混合物,对脂肪酶产生的影响很小,而添加0.9%的三油酸甘油酯可使牛奶中的脂肪酶活性降低90%。

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本文引用的文献

1
Synthesis of Extracellular Proteinase by Pseudomonas fluorescens Under Conditions of Limiting Carbon, Nitrogen, and Phosphate.荧光假单胞菌在碳、氮、磷限制条件下胞外蛋白酶的合成。
Appl Environ Microbiol. 1984 Jun;47(6):1224-7. doi: 10.1128/aem.47.6.1224-1227.1984.
2
The production and characterization of lipases from a micrococcus and a pseudomonad.
J Gen Microbiol. 1967 Sep;48(3):401-18. doi: 10.1099/00221287-48-3-401.
3
Microbial lipases: their characteristics, role in food spoilage and industrial uses.
J Dairy Res. 1986 Aug;53(3):481-505. doi: 10.1017/s0022029900025103.
4
Determination of the extracellular lipases of Pseudomonas fluorescens spp. in skim milk with the beta-naphthyl caprylate assay.用β-萘辛酸酯法测定荧光假单胞菌属在脱脂乳中的胞外脂肪酶。
J Dairy Res. 1986 May;53(2):301-12. doi: 10.1017/s0022029900024900.